Termite nest locations, excavation, and x-ray tomographic imaging

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Fig. 1 Termite nest locations, excavation, and x-ray tomographic imaging. Termite nest locations, excavation, and x-ray tomographic imaging. (A) Two sampling locations, Nguekokh (Senegal) and Kankan (Guinea)—marked by yellow circles, were selected for this study. The Senegal nest was taken from a region that is sandy on the surface (brownish in the satellite map), whereas the Guinea sample was taken from a more vegetated region. The map in this image is taken from Google Maps, with the data provider listed at the base of the image (Imagery 2017 Landsat/Copernicus, Data Scripps Institution of Oceanography, National Oceanic and Atmospheric Administration, U.S. Navy, National Geospatial-Intelligence Agency, General Bathymetric Chart of the Oceans, Map data 2017 Google). (B and C) Nests in the field in Senegal (B) and Guinea (C). (D and E) These nests were excavated from the field and brought to the laboratory for x-ray imaging. The yellow dashed lines in (D) and (E) show the boundary between the upperground and the underground portions of the nests. [Photo credit for parts (B) to (E): Christian Jost]. (F and G) The excavated nests were imaged in three dimensions nondestructively with a medical x-ray tomography scanner at a pixel resolution of 0.3 to 0.6 mm. The imaging plane was clipped vertically to show the interiors of both nests. Red and yellow represent solid material and the inner channels of the nest, respectively. (H) Histograms of the thickness of the outer walls of the nests, which was measured from the tomographic images (F) and (G) in the upper parts of the nests that were exposed to the atmosphere. Kamaljit Singh et al. Sci Adv 2019;5:eaat8520 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).