Superior and specific antitumor responses exhibited by SmarT cells. Superior and specific antitumor responses exhibited by SmarT cells. A, Schematic of animal model where NSG mice were engrafted with dual tumors—CAPAN-1 PSCA only (left flank) and CAPAN-1 PSCA + TGFβ + IL4 + (right flank)—and treated with SmarT cells labeled with firefly luciferase (FFluc+). B, Representative mice images and (C, D) quantification of SmarT-cell signal detected by bioluminescence imaging (n = 5–6 replicates per experiment with a maximum SEM of 0.01E+09 and 0.4E+09 photons/sec in the PSCA and PSCA + IL4 + TGFβ groups on day 18, respectively. However, the error bars are too small to be appreciated in C. E and F, Antitumor responses determined by calipers to assess tumor volume. (Although the maximum error bars for the PSCA and PSCA + IL4 + TGFβ groups are 96 and 29 mm3 on days 47 and 12, respectively, they are too small to be appreciated in E and F.) G and H, Superimposition of SmarT-cell signal against tumor volume. Data represent mean ± SEM (n = 5–6 mice per group). Sujita Sukumaran et al. Cancer Discov 2018;8:972-987 ©2018 by American Association for Cancer Research