Characterization of the carbohydrate preparations.

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Characterization of the carbohydrate preparations. Characterization of the carbohydrate preparations. (a) HPAEC-PAD traces showing the manno-oligosaccharides (degree of polymerization [DP], between 2 and 10) and manno-polysaccharides (MPS) (DP, >10) present in the AcGGM preparation. The AcGGM sample was chromatographed with the following external standards: M1, mannose; M2, mannobiose; M3, mannotriose; M4, mannotetraose; M5, mannopentaose; M6, mannohexaose. (b and c) MALDI-TOF spectra of low-molecular-weight (b) to medium-molecular-weight (c) components present in the AcGGM preparation. Masses of the sodium adduct ion (Mass + Na+) are shown. (d) HPAEC-PAD traces showing the xylo-oligosaccharides (DP ranging from 2 to 10) and xylo-polysaccharides (XPS) (DP, >10) present in the AcAGX preparation. Samples were analyzed with the following external standards: X1, xylose; X2, xylobiose; X3, xylotriose; X4, xylotetraose; X5, xylopentaose; X6, xylohexaose. (e and f) MALDI-TOF spectra of low-molecular-weight (e) to medium-molecular-weight (f) components present in the AcAGX preparation. Spectra show sodium adducts (Mass + Na+). In panels b, c, d, and e, the following abbreviations are used: Ac, acetyl group; Hex, hexose; Me, methyl group; GlcA, glucuronic acid; Xyl, xylose. Sabina Leanti La Rosa et al. mSphere 2019; doi:10.1128/mSphere.00554-18