Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD

Slides:

Advertisements

Similar presentations

Different signalling pathways regulate VEGF and IL-8 expression in breast cancer: implications for therapy Dina Chelouche-Lev, Claudia P. Miller, Carmen.

Advertisements

Patients with ulcerative colitis responding to steroid treatment up-regulate glucocorticoid receptor levels in colorectal mucosa L. Flood, E. Innala,

Microwave Ablation for Atrial Fibrillation: Dose-Response Curves in the Cardioplegia- Arrested and Beating Heart Sydney L. Gaynor, MD, Gregory D. Byrd,

Effects of pleiotrophin, a heparin-binding growth factor, on human primary and immortalized chondrocytes Dr. T. Pufe, Ph.D., G. Groth, M.B. Goldring,

Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription.

L. J. Sandell, Ph. D. , X. Xing, M. D. , C. Franz, M. A. , S

Volume 59, Issue 3, Pages (March 2001)

Modulation by aspirin of nuclear phospho–signal transducer and activator of transcription 6 expression: Possible role in therapeutic benefit associated.

Epigenetic regulation of established human type 1 versus type 2 cytokine responses Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.

George D. Webb, PhD, Lay Har Lim, BSc, Vernon M. S

Acidosis-induced apoptosis in human and porcine heart

Celsior versus custodiol: early postischemic recovery after cardioplegia and ischemia at 5°C Juergen Ackemann, MD, Wolfgang Gross, PhD, Martin Mory,

Volume 118, Issue 4, Pages (April 2000)

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Constitutive and tumor necrosis factor-α-induced activation of nuclear factor-κB in adenomyosis and its inhibition by andrographolide Bin Li, M.S., Ming.

Kwo-Yih Yeh*,‡, Mary Yeh*, Jonathan Glass*, D.Neil Granger‡

Revascularization of severe hibernating myocardium in the beating heart: early hemodynamic and metabolic features Evasio Pasini, MD, Gianna Ferrari,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes Dr C. Lianxu, Ph.D.,

Vein Graft Harvesting Induces Inflammation and Impairs Vessel Reactivity Kazuhiro Hinokiyama, MD, Guro Valen, MD, PhD, Shinichi Tokuno, MD, PhD, Jenny.

Warm-blood cardioplegic arrest induces selective mitochondrial translocation of protein kinase Cε followed by interaction with 6.1 inwardly rectifying.

EPS15R, TASP1, and PRPF3 Are Novel Disease Candidate Genes Targeted by HNF4α Splice Variants in Hepatocellular Carcinomas Monika Niehof, Jürgen Borlak

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes N. Chabane, M.Sc.,

Diabetic hearts have lower basal urocortin levels that fail to increase after cardioplegic arrest: Association with increased apoptosis and postsurgical.

Ectopia cordis and other midline defects

P38 Mitogen Activated Protein Kinase Mediates Both Death Signaling and Functional Depression in the Heart Meijing Wang, MD, Ben M. Tsai, MD, Mark W.

Sp1 Is Required for Glucose-Induced Transcriptional Regulation of Mouse Vesicular Glutamate Transporter 2 Gene Tao Li, Liqun Bai, Jing Li, Suzu Igarashi,

Modulation of the expression of tissue plasminogen activator and its inhibitor by hypoxia in human peritoneal and adhesion fibroblasts Ghassan M Saed,

R Largo, Ph. D. , M. A Alvarez-Soria, B. S. , I Dı́ez-Ortego, B. S

The role of cardioplegia induction temperature and amino acid enrichment in neonatal myocardial protection Michael T Kronon, MD, Bradley S Allen, MD,

Gene expression of inflammatory mediators in different chambers of the human heart Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Anna M Bennet, Göran.

Differentiation potential of human muscle-derived cells towards chondrogenic phenotype in alginate beads culture R. Andriamanalijaona, Ph.D., E. Duval,

Ischemic preconditioning is not cardioprotective in senescent human myocardium Babett Bartling, PhD, Ivar Friedrich, MD, Rolf-E Silber, MD, Andreas Simm,

Superiority of magnesium cardioplegia in neonatal myocardial protection Michael T Kronon, MD, Bradley S Allen, MD, Janeen Hernan, MS, Ari O Halldorsson,

Role of apoptosis in myocardial stunning after open heart surgery

Inhibition of protein kinase Cα improves myocardial β-adrenergic receptor signaling and ventricular function in a model of myocardial preservation Karen.

Fibronectin promotes VEGF-induced CD34+ cell differentiation into endothelial cells Errol S Wijelath, PhD, Salman Rahman, PhD, Jacqueline Murray, PhD,

Daniel R. Meldrum, MD, Brian D

Improved profile of bad phosphorylation and caspase 3 activation after blood versus crystalloid cardioplegia Jun Feng, MD, PhD, Cesario Bianchi, MD,

Glucocorticoids reduce ischemia-reperfusion-induced myocardial apoptosis in immature hearts Jeffrey M Pearl, MD, David P Nelson, MD, PhD, Steven M Schwartz,

Molecular targets of gene therapy

Cardioplegia and Diazoxide Modulate STAT3 Activation and DNA Binding

Studies of hypoxemic/reoxygenation injury with aortic clamping

Expression of Nuclear Factor-κB and Its Clinical Significance in Nonsmall-Cell Lung Cancer Zhenfa Zhang, MD, Jianqun Ma, MD, Ni Li, PhD, Nan Sun, MD,

Hermann Aebert, MD, Torsten Cornelius, BS, Tobias Ehr, BS, Stephan R

Moderate hypothermia during cardiopulmonary bypass increases intramyocardial synthesis of heat shock protein 72 Ma Qing, MD, Jaime F. Vazquez-Jimenez,

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Minimally Invasive Fibrillating Heart Surgery: A Safe and Effective Approach for Mitral Valve and Surgical Ablation for Atrial Fibrillation Paul S. Massimiano,

Leslie A. Bruggeman, Scott H. Adler, Paul E. Klotman

Coronary Microvascular Reactivity After Ischemic Cold Storage and Reperfusion Charles O Murphy, MD, Pan- Chih, MD, John Parker Gott, MD, Robert A Guyton,

Volume 8, Issue 2, Pages (February 1998)

Volume 62, Issue 3, Pages (September 2002)

Comparative effects of IL-1β and hydrogen peroxide (H2O2) on catabolic and anabolic gene expression in juvenile bovine chondrocytes G. Martin, Ph.D.,

Human angiopoietin gene expression is a marker for severity of pulmonary hypertension in patients undergoing pulmonary thromboendarterectomy Patricia.

Importance of endothelial nitric oxide synthase for the hypothermic protection of lungs against ischemia-reperfusion injury Li Zhang, MD, Sanjeev Kumar,

Editorial Board, January 2010

Klotho is a target gene of PPAR-γ

Jun Feng, MD, PhD, Hongling Li, MD, MSc, Eliot R Rosenkranz, MD

Markers for endothelial activation during open heart surgery

Adenosine-enhanced ischemic preconditioning provides myocardial protection equal to that of cold blood cardioplegia James D McCully, PhD, Masahisa Uematsu,

Endobronchial transfection of naked TGF-β1 cDNA attenuates acute lung rejection Franco D’Ovidio, MD, Motoki Yano, MD, Jon H Ritter, MD, Thalachallour.

Anthony P.C Yim, MD, Song Wan, MD, PhD, Tak Wai Lee, Ahmed A Arifi

Sinus mucosal IL-8 gene expression in chronic rhinosinusitis

Simultaneous Antegrade And Retrograde Delivery Of Continuous Warm Blood Cardioplegia After Global Ischemia Anders B. Ericsson, MD, Shigeto Takeshima,

Self-managed anticoagulation: results from a two-year prospective randomized trial with heart valve patients Pushpinder Sidhu, Hugh O O’Kane The Annals.

Gene expression profile after cardiopulmonary bypass and cardioplegic arrest Marc Ruel, MD, MPH, Cesario Bianchi, MD, PhD, Tanveer A Khan, MD, Shu Xu,

Arrest Duration Influences Postcardioplegia Electrophysiologic Recovery and Reperfusion Arrhythmias C.Patrick Murrah, Edward R Ferguson, Russell D Spruell,

Increased Rehospitalization Rate After Coronary Bypass Operation for Acute Coronary Syndrome: A Prospective Study in 200 Patients Staffan Bjessmo, MD,

Mark I. Block, MD The Annals of Thoracic Surgery

Self-Management of Oral Anticoagulation Therapy Improves Long-Term Survival in Patients With Mechanical Heart Valve Replacement Heinrich Koertke, MD,

Myocardial perfusion during warm antegrade and retrograde cardioplegia: a contrast echo study Michael A Borger, MD, Kevin S Wei, MD, Richard D Weisel,

Presentation transcript:

Induction of inflammatory mediators during reperfusion of the human heart Guro Valen, MD, PhD, Gabrielle Paulsson, PhD, Jarle Vaage, MD, PhD The Annals of Thoracic Surgery Volume 71, Issue 1, Pages 226-232 (January 2001) DOI: 10.1016/S0003-4975(00)02146-9

Fig 1 Polyacrylamide gel with RT-PCR products of left ventricular biopsies of 1 patient with unstable angina scheduled for acute CABG. [33P]dATP was incorporated to detect PCR products in the linear phase of the PCR. In left ventricular biopsies before cardioplegia (B) and after reperfusion (A), primers for housekeeping gene histone H3 (215 bp), E-selectin (CD62E, 255 bp), eNOS (737 bp), IL-1β (464 bp), TNFα (444 bp), CD25 (312 bp), ICAM-1 (237 bp), CD18 (379 bp), iNOS (532 bp), and ET-1 (725 bp) were added. The ratios between H3 and test genes were calculated to evaluate changes in mRNA. The Annals of Thoracic Surgery 2001 71, 226-232DOI: (10.1016/S0003-4975(00)02146-9)

Fig 2 Changes in gene expression in left ventricular biopsies of 19 patients undergoing open heart surgery with cardioplegic arrest. The factor increase or decrease during reperfusion is calculated as ratio test gene/reference gene during reperfusion/ratio before cardioplegia. The effect of cardioplegia-reperfusion on E-selectin (CD62E), eNOS, IL-1β, TNFα, CD25, ICAM-1, CD18, iNOS, and ET-1 are shown (mean ± SEM). ∗p < 0.05 compared with initial expression. The Annals of Thoracic Surgery 2001 71, 226-232DOI: (10.1016/S0003-4975(00)02146-9)

Fig 3 Changes in gene expression in left ventricular biopsies of 7 patients with unstable angina (UA) and 7 patients with stable angina (SA) after cardioplegic arrest and reperfusion (see also text to Fig 2) (mean ± SEM). ∗p < 0.05 compared with initial expression. The Annals of Thoracic Surgery 2001 71, 226-232DOI: (10.1016/S0003-4975(00)02146-9)

Fig 4 Identification of NFκB complexes in nuclear extracts from human right and left atrial tissue sampled during open heart surgery. The extracts were resolved by EMSA as described in Material and Methods. Extracts from right atrial auricles taken before cardioplegia (B) and from left atrial auricles after reperfusion (A) are shown in 2 patients (nos. 1 and 2). Supershifts were performed with antibodies against the p50 and p65 subunits of NFκB on the A samples. Cold probe competition on the two A samples using a 25- (25cp) or 50-fold (50cp) excess of unlabeled probe abolished the bands, whereas they were not influenced by a 25- or 50-fold excess of an unlabeled AP-1 probe (25AP, 50AP). The Annals of Thoracic Surgery 2001 71, 226-232DOI: (10.1016/S0003-4975(00)02146-9)

Fig 5 The optical density of NFκB bands taken before cardioplegia (B) and after reperfusion (A) in 10 patients. Mean ± SEM values are shown. ∗p < 0.0001. The Annals of Thoracic Surgery 2001 71, 226-232DOI: (10.1016/S0003-4975(00)02146-9)