Involvement of FKHR (FOXO1) transcription factor in human uterus leiomyoma growth  Kálmán A. Kovács, M.D., Ph.D., Ferenc Lengyel, Ph.D., Ferenc Wilhelm, M.D., Zsuzsanna Vértes, M.D., Ph.D., Balazs Sumegi, D.Sc., Marietta Vértes, M.D., D.Sc.  Fertility and Sterility  Volume 94, Issue 4, Pages 1491-1495 (September 2010) DOI: 10.1016/j.fertnstert.2009.07.1670 Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Changes of total FOXO1 (A) and pSer256-FOXO1 protein (B) level in leiomyoma and adjacent myometrium during menstrual cycle and at menopause. Bars, represent mean ± SE of data. Black bars represent adjacent myometrium, and open bars represent leiomyoma. The Relative density (ordinates) is the density of studied protein/density of actin. Inserts show representative blots. Western blot analyses were made at least three times in three independent tissue preparations with comparable results. Actin is shown as a load control. Asterisks indicate statistically significant differences at P < 0.05: ∗leiomyoma vs. corresponding myometrium. P, proliferative phase (n = 19); S, secretory phase (n = 19); Meno, menopause (n = 20). Fertility and Sterility 2010 94, 1491-1495DOI: (10.1016/j.fertnstert.2009.07.1670) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Changes of pSer473-Akt/Akt ratio in leiomyoma and adjacent myometrium during menstrual cycle and at menopause. Bars represent mean ± SE of data. Black bars represent adjacent myometrium; open bars represent leiomyoma. Relative density (ordinate) is the density of pAkt/density of total Akt; both of them are corrected by loading controls. Inserts show representative blots. Western blot analyses were made at least three times in three independent tissue preparations with comparable results. Asterisks indicate statistically significant differences at P < 0.05. ∗Leiomyoma vs. corresponding myometrium; P, proliferative phase (n = 19); S, secretory phase (n = 19); Meno, menopause (n = 20). Fertility and Sterility 2010 94, 1491-1495DOI: (10.1016/j.fertnstert.2009.07.1670) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Subcellular distribution of pSer256-FOXO1 protein in cytoplasmic (A) and in nuclear-rich (B) fractions of leiomyoma and adjacent myometrium during the menstrual cycle and at menopause. Bars represent mean ± SE of data. Black bars represent host myometrium of leiomyoma; open bars represent leiomyoma. Inserts show representative blots. Relative density (ordinates) is the density of studied protein/density of actin. Western blot analyses were made at least three times in three independent tissue preparations with comparable results. Asterisks indicate statistically significant differences at P < 0.05. ∗Leiomyoma vs. corresponding myometrium; P, proliferative phase; S, secretory phase; Meno, menopause. Fertility and Sterility 2010 94, 1491-1495DOI: (10.1016/j.fertnstert.2009.07.1670) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

Figure 4 Changes of 14-3-3 γ protein level in leiomyoma and adjacent myometrium during menstrual cycle and at menopause. Bars represent mean ± SE of data. Black bars represent host myometrium of leiomyoma; open bars represent leiomyoma. Relative density (ordinates) is the density of studied protein/density of actin. Inserts show representative blots. Western blot analyses were made at least three times in three independent tissue preparations with comparable results. Asterisks indicate statistically significant differences at P < 0.05 level. ∗Leiomyoma vs. corresponding myometrium; P, proliferative phase (n = 19); S, secretory phase (n = 19); Meno, menopause (n = 20). Fertility and Sterility 2010 94, 1491-1495DOI: (10.1016/j.fertnstert.2009.07.1670) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions