Expression of anti-versican shRNA decreased breast cancer cell mammosphere and colony formation. Expression of anti-versican shRNA decreased breast cancer.

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Expression of anti-versican shRNA decreased breast cancer cell mammosphere and colony formation. Expression of anti-versican shRNA decreased breast cancer cell mammosphere and colony formation. A, typical mammospheres of vector- and anti-versican shRNA-transfected MDA-MB-231 cells cultured in suspension using serum-free medium. B, numbers of small and large mammospheres. Compared with vector control group; *, P < 0.05; **, P < 0.01; n = 5; analyzed with t test. C, the typical prevalence of Hoechstlow side population (SP) cells of vector- and anti-versican shRNA-transfected MDA-MB-231 cells shown by flow cytometry. D, the vector- and anti-versican shRNA-transfected MDA-MB-231 cells were cultured in 10%FBS/DMEM medium, lysised, and subjected to immunoblotting with antibodies to versican V1, pEGFR, pAKT, GSK-3β (S9P), Sca-1, Sox2, ALDH1, and β-actin. E, typical colonies of vector- and anti-versican shRNA-transfected MDA-MB-231 cells formed in soft agarose gel after culture for 4 weeks. F, the numbers of colonies formed on soft agarose gel. Compared with vector control group; *, P < 0.05; **, P < 0.01; n = 3; analyzed with t test. William Weidong Du et al. Mol Cancer Res 2013;11:443-455 ©2013 by American Association for Cancer Research