Quantitative PCR (A) and Western blot (B) analyses for expression of CD133 and ALDH isoforms in CD133+ and CD133− cells sorted from HCC cell lines PLC8024.

Slides:

Advertisements

Similar presentations

- KLF6 869 bp IHH HepG2 Hep3B HuH bp- 800 bp- 700 bp- 600 bp- SV1 715 bp SV2 743 bp SV3 745 bp IHH HepG2 Hep3B HuH Percentage of.

Advertisements

Laser Flow Cytometry Forward Scatter indicates size Forward Scatter.

The expression and signaling of FGFR3 in WM cells.

Enhanced DNA damage in IDH1 mutant glioma cells.

Expression of adenylyl cyclases and miR‐142‐3p in CD4+CD25− T cells and CD4+CD25+ regulatory T cells. Expression of adenylyl cyclases and miR‐142‐3p in.

Human articular chondrocytes with higher aldehyde dehydrogenase activity have stronger expression of COL2A1 and SOX9 A. Unguryte, E. Bernotiene, E. Bagdonas,

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

Volume 132, Issue 7, Pages (June 2007)

Flow cytometric analysis of putative progenitor cells.

Flow cytometry analysis of HLA-B27 and HLA-B7 transduced cells.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

Volume 10, Issue 5, Pages (May 2018)

Truncated protein isoforms of CD19 provide proliferative advantage.

Overexpression of HGF decreases MET protein levels.

CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

Comparison of conventional NSG and hIL-7 expressing NSG humanized mice

Relationship between blood cell and plasma miRNA expression among published circulating cancer biomarkers. Relationship between blood cell and plasma miRNA.

A B C D E F Supplementary Fig. 3. Gating strategy for ES2 ALDHhi tumor initiating cells (TIC). Cells were treated with the specific aldehyde dehydrogenase.

IL-6 inhibits insulin-induced formation of p85/IRS-1 complexes.

CYP1A1 knockdown increases cell death.

Aberrant regulation of HDAC2 and its association with malignant proliferation of human gastric cancer. Aberrant regulation of HDAC2 and its association.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

VHL-, oxygen-, and proteasome-dependent regulation of AUF1 expression in RCC and 293T cells. VHL-, oxygen-, and proteasome-dependent regulation of AUF1.

Variation in dUTPase expression in cell lines.

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Silencing hOGG1 triggers caspase-3 and caspase-7 activation in response to H2O2 in GM00637 cells. Silencing hOGG1 triggers caspase-3 and caspase-7 activation.

BME treatment increases granzyme B expression in NK 3.3 cells.

Colony-forming capacity of CD34+ cells with and without CLL-1 expression. Colony-forming capacity of CD34+ cells with and without CLL-1 expression. CD34+

ALDH1A3 is mainly responsible for the ALDH activity in two human cholangiocarcinoma lines. ALDH1A3 is mainly responsible for the ALDH activity in two human.

JMJD2B and CDK6 expressions are significantly correlated in various types of cell lines. JMJD2B and CDK6 expressions are significantly correlated in various.

Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. Mitotic catastrophe symptoms caused by curcumin are followed by apoptosis. A.

Ki-67 expression is dynamically controlled by a cell-cycle–regulatory network. Ki-67 expression is dynamically controlled by a cell-cycle–regulatory network.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

A, one-step viral growth curves: RT treatment increased the virus yield in MV-CEA–treated U87 cells by up to 2 log as compared with MV-CEA infection only.

Depletion of UCP1 and Myf5 population reduced xenograft growth.

Expression of CRC stem cell markers and L1 in CRC cells.

Melanoma patient monocytes have altered expression of inflammatory and surface markers. Melanoma patient monocytes have altered expression of inflammatory.

Effect of TLR-4 ligation with LPS on NF-κB activation in EOC cells: (A) MyD88+ and (B) MyD88− EOC cells were treated with 10 μg/mL LPS for 1, 2, and 4.

A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety of 24 human cell lines. A, RT-PCR expression of matriptase-1 and matriptase-2 in a variety.

Src expression in a panel of human TCC cell lines.

Expression pattern of TLR-4 and MyD88 in EOC cells.

Staurosporine induces changes in MMP

COX-2, Oct-4, and ALDH-1 expression in the mammary gland.

Bim expression and activation correlates with reduced Notch3 expression and increased anoikis. Bim expression and activation correlates with reduced Notch3.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

BCG uptake by bladder cancer cells requires activated Pak1.

Reduced klotho expression in pancreatic cancer.

Characterization of DNA-PK and PARP-1 levels and activities in the cell lines studied. Characterization of DNA-PK and PARP-1 levels and activities in the.

Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.

DAC treatment alters immune cell composition and enhances cytokine production in the peritoneal lavage. DAC treatment alters immune cell composition and.

Tivantinib does not inhibit MET function.

Substitution of endogenous Reptin with D299N mutant leads to HuH7 cell growth arrest. Substitution of endogenous Reptin with D299N mutant leads to HuH7.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and HAL-treated xenografts. Expression of HIF-1α and β-catenin in hypoxic HCC cell lines and.

DCRunx3−/− mice resistance to DMBA/TPA–induced tumorigenesis.

Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3 S284 through MDM2. Cell-cycle regulatory proteins were controlled by O-GlcNAc at FOXO3.

TGFβ1/Smad3 signaling suppresses IFNγ production via an E4BP4-dependent mechanism in NK-92 cells. TGFβ1/Smad3 signaling suppresses IFNγ production via.

P53 restoration in a syngeneic transplant model of Mdm2Tg p53Neo/Neo CreER angiosarcoma. p53 restoration in a syngeneic transplant model of Mdm2Tg p53Neo/Neo.

W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells. W. chinensis extract induces apoptosis in AR-dependent prostate cancer cells.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

Effect of BCAA on the progression of cell cycle, expression of p21CIP1, and induction of apoptosis in HepG2 cells in the presence and absence of visfatin.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

Effects of NMT siRNAs on MARCKS and c-Src.

Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced proliferation in HepG2 cells. Effects of inhibitors of PI3K, MEK1, and GSK-3β on visfatin-induced.

Proteome changes associated with CHCHD2 expression in NSCLC cells.

Overexpression of SUFU and GLI1 in the poor clinical outcome subgroup was confirmed by real-time PCR along with its association with disease progression.

Phenotyping of T cells. Phenotyping of T cells. Circulating lymphocytes from a tumor-naïve C57BL/6 mice (left column) were compared with those from C57BL/6.

Silencing E4BP4 abrogates the promoting effect of SMAD3 knockdown on IFNγ production in NK-92 cells. Silencing E4BP4 abrogates the promoting effect of.

Presentation transcript:

Quantitative PCR (A) and Western blot (B) analyses for expression of CD133 and ALDH isoforms in CD133+ and CD133− cells sorted from HCC cell lines PLC8024 and Huh7. Quantitative PCR (A) and Western blot (B) analyses for expression of CD133 and ALDH isoforms in CD133+ and CD133− cells sorted from HCC cell lines PLC8024 and Huh7. ALDH1A1, along with several other ALDH isoforms, was found to be preferentially expressed in the CD133+ subpopulation. C. Quantitative PCR analysis for expression of ALDH1A1 in a panel of liver cell lines. Expression of ALDH1A1 in the liver cell lines directly correlated with their expression of CD133. D. Representative flow cytometry analyses of hepatoblastoma cell line HepG2 and HCC cell lines PLC8024 and Hep3B for ALDH activity using the ALDEFLUOR Kit (Stem Cell Technologies). Left, negative controls when the cells were treated with the ALDH inhibitor diethylaminobenzaldehyde (DEAB). SSC-H, side scatter; FL1-H, FITC channel or ALDH activity. Those cells gated in R2 represent the subpopulation of HCC cells that are positive for ALDH activity. Stephanie Ma et al. Mol Cancer Res 2008;6:1146-1153 ©2008 by American Association for Cancer Research