Genomic and proteomic profiling of ovarian cancer cell lines.

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Genomic and proteomic profiling of ovarian cancer cell lines. Genomic and proteomic profiling of ovarian cancer cell lines. A, probe-level (blue dots) and segmentation data (red lines) from Agilent 244K arrays identifying representative focal amplification (KRAS in SKOV-8) and focal homozygous deletion (RB1 in SKOV-433) events. The vertical green line crosses the x-axis at the chromosomal position of the gene. Y-axis indicates log2 copy number signal. B, immunoblot analysis of the ovarian cancer cell line panel for expression of AKT1/2/3, phosphorylated AKT (Ser473), and activation and abundance of key downstream targets. Cells were arranged based on the presence of PI3K/AKT (red) or RAS/RAF-pathway alterations (blue) and RB1 loss (green and green underline). Aphrothiti J. Hanrahan et al. Cancer Discov 2012;2:56-67 ©2012 by American Association for Cancer Research