GK1 enhances the secretion of proinflammatory cytokines resulting from cognate interactions between activated DCs and T cells. GK1 enhances the secretion of proinflammatory cytokines resulting from cognate interactions between activated DCs and T cells. Supernatants from the cocultures of LPS-treated DCs or LPS- and GK1-treated DCs obtained from the experiment whose results are shown in Fig. 2B were recovered, and the cytokine levels were determined by flow cytometry with a cytometric bead array mouse inflammation kit (BD Biosciences). The results represent the mean of two independent experiments. René Segura-Velázquez et al. Clin. Vaccine Immunol. 2009; doi:10.1128/CVI.00106-09