Volume 19, Issue 12, Pages 2462-2468 (June 2017) The Sensory Striatum Is Permanently Impaired by Transient Developmental Deprivation  Todd M. Mowery, Kristina B. Penikis, Stephen K. Young, Christopher E. Ferrer, Vibhakar C. Kotak, Dan H. Sanes  Cell Reports  Volume 19, Issue 12, Pages 2462-2468 (June 2017) DOI: 10.1016/j.celrep.2017.05.083 Copyright © 2017 The Author(s) Terms and Conditions

Cell Reports 2017 19, 2462-2468DOI: (10.1016/j.celrep.2017.05.083) Copyright © 2017 The Author(s) Terms and Conditions

Figure 1 Anatomical and Functional Mapping of the Auditory Corticostriatal Projection (A) Fluorescence image showing the ACx injection site and retrograde labeling in the medial geniculate nucleus (MG). Scale bar, 25 μm. (B) Fluorescence image of a coronal slice showing dense anterograde labeling from the ACx to the dorsolateral striatum. Scale bar, 1 mm; inset, 25 μm. (C) Fluorescence image of a coronal slice containing sparse ACx labeling within the dorsomedial striatum. Scale bar, 1 mm; inset, 25 μm. (D) Scatterplot showing the percentage of total area within the striatum labeled by ACx injections (y axis) as a function of distance from the rostral pole of the hippocampus (x axis). (E) Diagram showing the ACx site of AAV injection and the plane of the slice preparation containing the injection site. (F) Schematic of the corticostriatal slice preparation with the ACx L5/6 injection site and labeled pathways. (G) Fluorescence image showing ACx L5/6 injection site and labeling in the MG but no labeling in the lateral geniculate nucleus (LG). (H) Fluorescence image showing anterograde labeled fibers of passage and terminals from L5 pyramidal neurons synapsing onto striatal neurons (inset). (I) Diagram showing the slice preparation and experimental design for evoking PSPs in striatal neurons. (J) Micrograph showing the region of the striatum used for functional mapping. Numbers represent the site of a patched neuron and are accompanied by traces of PSPs following stimulation of L5. (K) Micrograph showing the approximate mediolateral-rostrocaudal location of recorded striatal neurons used for functional mapping and the peak amplitude of the PSP for each cell (colored bar, left). (L) A scatter diagram showing the peak PSP amplitude as a function of distance from the rostral pole of the hippocampus. Cell Reports 2017 19, 2462-2468DOI: (10.1016/j.celrep.2017.05.083) Copyright © 2017 The Author(s) Terms and Conditions

Figure 2 The Effect of Transient Developmental HL on Cortical and Striatal Intrinsic Properties (A) Diagram illustrating the experimental design (top), and examples of a recorded L5 pyramidal neuron (bottom left) and a recorded medium spiny neuron (bottom right) filled with fluorescent biotin. (B) Bar graph showing the average resting membrane potential in control (Ctl) and HL animals for L5 pyramidal neurons (L5, orange) and striatal MSNs (Str, green). (C) Bar graph showing the average membrane resistance in response to a −30 pA current pulse in control and HL animals for L5 pyramidal neurons and striatal MSNs. (D) Representative traces are shown for a Ctl and an HL L5 neuron in response to depolarizing and hyperpolarizing current pulses (left). The plot compares the average input-output functions for ACx L5 neurons in Ctl and HL animals. The Ctl:HL ratio of the maximum firing rate at an injected current level of 600 pA was 0.94 (dashed box). (E) Representative traces are shown for a Ctl and an HL striatal MSN in response to depolarizing and hyperpolarizing current pulses (left). The plot compares the average input-output functions for striatal MSNs in Ctl and HL animals. The Ctl:HL ratio of the maximum firing rate at an injected current level of 600 pA was 0.51 (dashed box). Data are represented as mean ± SEM. ∗∗p < 0.01. Cell Reports 2017 19, 2462-2468DOI: (10.1016/j.celrep.2017.05.083) Copyright © 2017 The Author(s) Terms and Conditions

Figure 3 The Effect of Transient Developmental HL on Cortical and Striatal Inhibitory Gain (A) Representative traces showing L5 (top, orange) and striatal (bottom, green) sIPSCs from Ctl and HL animals. The bar graph shows average sIPSC amplitude in Ctl and HL animals for ACx L5 and striatal MSNs. (B) Representative traces showing L5 (top, orange) and striatal (bottom, green) me-IPSCs. The bar graph shows average me-IPSC amplitude in Ctl and HL animals for ACx L5 and striatal MSNs. (C) Representative traces showing L5 (top, orange) and striatal (bottom, green) evoked IPSCs at 2× the threshold stimulus current. The bar graph shows average IPSC amplitude in Ctl and HL animals for ACx L5 and striatal MSNs. We computed the Ctl:HL ratio of evoked IPSC amplitude. The value was 2.8 for L5 neurons and 0.52 for striatal MSNs (dashed boxes). Data are represented as mean ± SEM. ∗∗p < 0.01. ∗∗∗p < 0.001. Cell Reports 2017 19, 2462-2468DOI: (10.1016/j.celrep.2017.05.083) Copyright © 2017 The Author(s) Terms and Conditions

Figure 4 The Effect of Developmental HL on Cortical and Striatal Excitatory Properties (A) Diagram showing the experimental design for recording light-evoked EPSPs. (B) Examples of a patched striatal MSN (top) and an L5 pyramidal cell (bottom) receiving synaptic terminals from ACx L5 pyramidal cells transfected with channelrhodopsin and mCherry fluorescent protein. Insets show light-evoked responses for these cells. (C) The graph compares average light-evoked responses in ACx L5 neurons for Ctl and HL animals. The Ctl:HL ratio of the maximum evoked EPSP amplitude was 2.9 (dashed box). (D) The graph compares average light-evoked responses in striatal MSNs for Ctl and HL animals. The Ctl:HL ratio of the maximum evoked EPSP amplitude was 3.8 (dashed box). (E) Example traces showing light-evoked AMPA receptor-mediated EPSPs (top) recorded at −80 mV (black) and in the presence of AP5 (red). The bar graph shows the average maximum light-evoked AMPA receptor-mediated EPSP amplitude in L5 pyramidal neurons and striatal MSNs for Ctl and HL animals. Data are represented as mean ± SEM. ∗∗p < 0.01. Cell Reports 2017 19, 2462-2468DOI: (10.1016/j.celrep.2017.05.083) Copyright © 2017 The Author(s) Terms and Conditions