Microarray transcriptional heat map analysis of cell wall stimulon activation of hVISA/VISA (defined by population analysis and vancomycin broth MIC) relative.

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Microarray transcriptional heat map analysis of cell wall stimulon activation of hVISA/VISA (defined by population analysis and vancomycin broth MIC) relative to parental VSSA. Five isolate pairs are included (P1 to P5). Microarray transcriptional heat map analysis of cell wall stimulon activation of hVISA/VISA (defined by population analysis and vancomycin broth MIC) relative to parental VSSA. Five isolate pairs are included (P1 to P5). For each pair, the hVISA/VISA and VSSA strains were isolated from the same patient. The fold ratio of gene transcription for hVISA/VISA compared to VSSA was calculated from pooled microarray data using The Institute for Genomic Research S. aureus arrays. Divergent transcriptional patterns were observed for the five pairs and demonstrate that for some hVISA/VISA strains, the cell wall stimulon is activated compared to parental VSSA strains, while in others, it is not activated compared to parental strains. HP, hypothetical protein. (Adapted from reference 122, which was published under an open-access license agreement.)‏ Benjamin P. Howden et al. Clin. Microbiol. Rev. 2010; doi:10.1128/CMR.00042-09