Molecular Spies for Bioimaging—Fluorescent Protein-Based Probes Atsushi Miyawaki, Yusuke Niino  Molecular Cell  Volume 58, Issue 4, Pages 632-643 (May 2015) DOI: 10.1016/j.molcel.2015.03.002 Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 1 Schematics of FRET-Based GECIs, YCs (A) The original YC, including YC3.12. (B) YC with cp173YFP, including YC3.60. Molecular Cell 2015 58, 632-643DOI: (10.1016/j.molcel.2015.03.002) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 2 FRET Constructs (A and B) Bimolecular (A) and unimolecular (B) FRET. D, donor; A, acceptor. Violet, blue, and orange zigzag arrows indicate excitation light for the donor, emission from the donor, and emission from the acceptor, respectively. Molecular Cell 2015 58, 632-643DOI: (10.1016/j.molcel.2015.03.002) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 3 Schematics of Single-FP-Based GECIs (A) G-CaMP, GCaMP, and pericam. (B) Camgaroo. Molecular Cell 2015 58, 632-643DOI: (10.1016/j.molcel.2015.03.002) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 4 Anatomical Distribution of Activity Clusters in One Fish during a Visuomotor Behavior Top, whole-brain recording of activity using GCaMP5G reveals four temporal clusters. Bottom, schematic outlining relevant brain regions in the zebrafish larvae in a dorsal view. OT, optic tectum; OT spv, optic tectum stratum periventriculare; IO, inferior olive; Pt, pretectal area; Hab, habenula. Data adapted from Portugues et al. (2014). Molecular Cell 2015 58, 632-643DOI: (10.1016/j.molcel.2015.03.002) Copyright © 2015 Elsevier Inc. Terms and Conditions

Figure 5 Correlation of Cell-Density-Dependent Stochastic ERK Dynamics with Cell Proliferation (A) Montage FRET images of nuclei in three representative cells expressing nuclear-localized EKAREV with different cell densities. (B) Frequency of ERK activity pulses as a function of cell density. (C) Cell-density-dependent proliferation rate in cells analyzed by EdU incorporation. (D) Percentage of EdU-positive cells as a function of cell density. Data adapted from Aoki et al. (2013a). Molecular Cell 2015 58, 632-643DOI: (10.1016/j.molcel.2015.03.002) Copyright © 2015 Elsevier Inc. Terms and Conditions