Fig. 4 Light-induced TRPV1 activation promotes in vitro tubulogenesis in ECFC cultures. Light-induced TRPV1 activation promotes in vitro tubulogenesis.

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Fig. 4 Light-induced TRPV1 activation promotes in vitro tubulogenesis in ECFC cultures. Light-induced TRPV1 activation promotes in vitro tubulogenesis in ECFC cultures. (A to D) Representative optical images of in vitro tubular network of ECFCs subjected to long-term optical excitation seeded either on bare glass or on P3HT thin films and treated respectively with CPZ (A), RR (B), RN-1734 (C), and BAPTA-AM (D). Scale bars, 250 μm. (E to G) Relative variation of number of master segments (E), master junctions (F), and meshes (G) of ECFCs subjected to long-term optical excitation seeded on P3HT in the absence [control (CTRL)] and presence of 10 μM CPZ, 10 μM RR, 20 μM RN-1734 (RN-1734), and 30 μM BAPTA-AM (BAPTA). The results are represented as the means ± SEM of three different experiments conducted on cells harvested from three different donors. The significance of differences was evaluated with one-way ANOVA coupled with Dunnett’s post hoc test. *P < 0.05 and **P < 0.01. F. Lodola et al. Sci Adv 2019;5:eaav4620 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).