Fig. 5 Cytosolic delivery of toxic proteins.

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Fig. 5 Cytosolic delivery of toxic proteins. Cytosolic delivery of toxic proteins. (A) Cytosolic delivery of saporin, RNase A, and trypsin into cancer cells leads to ribosome inactivity, RNA degradation, and intracellular protein hydrolysis, respectively, and cell death. Viability of MDA-MB-231 cells treated with (B) saporin/P4 and (C) RNase A/P4, and (D) HeLa cells treated with trypsin/P4 complexes determined by MTT assay. The concentration of trypsin was 40 μg/ml. Free saporin, RNase A, and trypsin were tested as controls. The dose of P4 in the experiments of cytosolic delivery of saporin, RNase A, and trypsin were 0.6, 0.6, and 2.5 μg in each well, respectively. Chongyi Liu et al. Sci Adv 2019;5:eaaw8922 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).