Yinghui Ye, M. D. , Ph. D. , Chenming Xu, PhD. , Yuli Qian, B. Sc

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Presentation transcript:

Evaluation of human sperm function after being cryopreserved within the zona pellucida Yinghui Ye, M.D., Ph.D., Chenming Xu, PhD., Yuli Qian, B.Sc., Fan Jin, M.D., Hefeng Huang, M.D. Fertility and Sterility Volume 92, Issue 3, Pages 1002-1008 (September 2009) DOI: 10.1016/j.fertnstert.2008.07.1737 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Empty zona pellucida without (A) or with (B) injected spermatozoa. Fertility and Sterility 2009 92, 1002-1008DOI: (10.1016/j.fertnstert.2008.07.1737) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Assessment of sperm chromatin damage using acridine orange (AO) staining. Fluorescence images of spermatozoa from fresh (A) or post-thaw samples cryopreserved with method I (B) or method II (C) stained with AO. Spermatozoa with intact chromatin were stained green and spermatozoa with damaged chromatin were stained orange or red. (D) Effect of cryopreservation on sperm chromatin damage. Values are mean and bars are SEM (n = 15); ∗P<.05 versus control group. Fertility and Sterility 2009 92, 1002-1008DOI: (10.1016/j.fertnstert.2008.07.1737) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 3 Assessment of sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay. TUNEL assay applied to spermatozoa from fresh (A) or post-thaw samples cryopreserved with method I (B) or method II (C). Spermatozoa with or without DNA fragmentation showed green and blue fluorescence, respectively. (D) Effect of cryopreservation on sperm DNA fragmentation. Values are mean and bars are SEM (n = 12); ∗P<.05 versus control group. Fertility and Sterility 2009 92, 1002-1008DOI: (10.1016/j.fertnstert.2008.07.1737) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions