The expanded stem/progenitor niche in GFAP-Cre+/K-rasG12D mice brains was composed of distinct subpopulations based on cytology and protein expression.

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The expanded stem/progenitor niche in GFAP-Cre+/K-rasG12D mice brains was composed of distinct subpopulations based on cytology and protein expression. The expanded stem/progenitor niche in GFAP-Cre+/K-rasG12D mice brains was composed of distinct subpopulations based on cytology and protein expression. Adjacent, representative, parasagittal sections from two animals (A-B and C-E) are shown, stained with H&E (A and C. Original magnification, ×200) and antibody for GFAP (C and D; original magnification, ×200). An additional adjacent section stained with antibody to TUJ1 is shown (E. Original magnification, ×200). Cells with angular, hyperchromatic nuclei populated the region closest to the ventricle (bars in A and C). A second population of cells with smaller, round nuclei and more open chromatin was also present (brackets in A and C). The cells with dark, angular nuclei were immunoreactive for TUJ1, a marker of immature neurons (E), and they did not express GFAP (B and D). Conversely, the smaller cells were immunoreactive for GFAP (B and D) and did not express TUJ1 (E). Both cell types were highly proliferative, as indicated by Ki67 immunohistochemistry (F. Original magnification, ×200). The cells expressed Cre protein (G. Original magnification, ×200) and high levels of p-MAPK (H. Original magnification, ×200; v, ventricle), consistent, respectively, with expression of the GFAP-Cre transgene and activation of the Ras pathway in these cells. Note the absence of significant p-MAPK labeling outside the expanded SVZ/RMS (I and H). p-MAPK signal is low to undetectable in cortex from the same animal shown in H (I. Original magnification, ×200). Arrow, the ependymal layer in all panels. Ty W. Abel et al. Mol Cancer Res 2009;7:645-653 ©2009 by American Association for Cancer Research