Liquid Nitrogen Dewar Filling Station for safe use in confined work areas. Edwards S1, Arklay C1, Crameri S1, May A2, van Driel R1 and Hyatt AD1. 1 AAHL Biosecurity Microscopy Facility CSIRO, Livestock Industries, Australian Animal Health Laboratory, PO Bag 24 Geelong, Vic 3220 abmf@csiro.au. 2 Cryoquip Australia, 14 Zenith Road, VIC 3175, AUSTRALIA. 5 Introduction A Dewar Filling Station was developed for use inside buildings. Safety mechanisms include automatic Liquid Nitrogen (LN2) filling control, monitoring and shutdown systems. LN2 is commonly used in the microscopy laboratory environment yet it can cause a variety of significant risks to the health and safety of people. The risks include asphyxiation, pressure build up and explosion, splashing causing burns/frostbite and manual handling injuries. Several workplace fatalities have resulted from nitrogen exposures and one such incident occurred in 2001at CSIRO Livestock Industries’ Australian Animal Health Laboratory (AAHL). AAHL is a high security bio-containment facility having a number of airtight laboratories designed to contain biological agents. In this environment the risks associated with handling LN2 are increased. Even small spills or leaks have the potential to become life threatening as oxygen is rapidly displaced by the vaporising LN2. Our team consulted with a supplier of cryogenic handling systems, Cryoquip Australia, on possible design solutions. The outcome was a practical and effective engineering control. Figure 5: Overall view of LN2Dewar Filling Station. The dewar is placed on a metal tray, which is the active plate of a set of weighing scales. The metal tray is also equipped with a temperature sensor (white arrow), which will cease the flow of LN2 in the event of a spill. Dewar Filling Station Safety Features Weighing scale recessed into floor eliminating trip hazards when moving dewar in/out of the Filling Station. Barcode scanner reads unique barcode indentifying its individual size and weight. The filling can only commence when a known bar-coded dewar is in position. The station monitors and continues to fill until a pre-programmed weight is achieved. Safety Programmable Logic Controller (PLC) provides instructions for filling, the status of the fill process, and alarms with actions to be taken. Alarmed automatic shutoff valves close supply when a overflow or spill of LN2 is detected. Interlocks on filling hoses ensure correct filling hose is released for coded open or pressure dewars. Station door interlocks, monitored by the PLC, prevent filling if open. O2 monitors activate room lock out when O2 concentration falls below 20% in the room containing the Filling Station. Power failure protective shutdown. Emergency stop button allowing staff to manually cease the LN2 filling process. 1 2 3 4 Figure 1: Pressure dewar filling, demonstrates LN2 gas venting into ceiling duct. Shows filling valves (red tops), temperature sensor (silver), and gas vent valves (green). Figure 2: Entry procedure to LN2 filling room. Only trained personnel are permitted access in restricted hours. The room locks out when O2 level fall below 20%. Figure 3: Barcode scanner recognizes the individual dewar and its pre-programmed fill weight. Figure 4: Open dewar filling, demonstrates LN2 gas venting into ceiling duct direct from cyclone filling head on a pivot swing arm. contact: Steve Edwards phone: (03) 52275450 email: steven.edwards@csiro.au poster prepared by: Sandra Crameri Acknowledgements The authors would like to thank Clare Holmes1 and Andrew Leis1 for assistance with photography.