Expression levels of additional giardia genes in elutriation fractions

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Expression levels of additional giardia genes in elutriation fractions

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Expression levels of additional giardia genes in elutriation fractions. Expression levels of additional giardia genes in elutriation fractions. Data represent transcript levels of the giardia genes encoding (A) thymidine kinase (TK), (B) minichromosome maintenance 5 (MCM5), (C) polo-like kinase (PLK), and (D) proliferating cell nuclear antigen (PCNA). The average expression levels of these genes were calculated from four separate RT-qPCR analyses of fractions collected in two independent elutriation experiments. The RT-qPCR data were normalized to the actin-related gene, and the expression levels are shown relative to those determined for fraction 5 as the calibrator. The G1/S and G2 bars above the graph denote the predominant cell type in the fractions according to FC analysis results. Kathleen Horlock-Roberts et al. mSphere 2017; doi:10.1128/mSphere.00384-16