miR-181a knockdown inhibits VEGF and MMP1 secretion in vitro.

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miR-181a knockdown inhibits VEGF and MMP1 secretion in vitro. miR-181a knockdown inhibits VEGF and MMP1 secretion in vitro. VEGF and pro-MMP1 in CM were analyzed with ELISA and Western blotting, as described in Materials and Methods. A and B, CS-1 cells were transfected with anti–miR-181a at various concentrations and VEGF and MMP1 in CM measured after 2 days. C and D, CS-1 cells were transfected with 40 nmol/L anti–miR-181a and VEGF and MMP1 were measured for 3 days with ELISA and on day 2 with Western blotting (E). CS-1 cells (F and G) and JJ cells (H and I) were transfected with miR-181a or control or lentivirus expression construct with anti–miR-181a or control and VEGF and MMP1 concentration measured 2 days after transfection; *, P < 0.001; #, P < 0.01. Xiaojuan Sun et al. Mol Cancer Res 2015;13:1347-1357 ©2015 by American Association for Cancer Research