The Microscope.

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Presentation transcript:

The Microscope

Microscopy Type of Microscopy is the technology of making very small things visible to the human eye . Type of Microscopy 1-Light Microscopes one of the most useful, and often used type of microscope available to the microbiologist 2-Electron Microscopes use electrons instead of photons (light) to illuminate a specimen

Bright-Field Microscope Microscopy Light Microscopes Bright-Field Microscope Dark-Field Microscope Phase-Contrast Microscope Fluorescence Microscope Electron Microscopes Transmission Electron Microscope

Parts and Specifications Eyepiece Lens the lens at the top that you look through. They are usually 10X or 15X power. Tube Connects the eyepiece to the objective lenses Arm Supports the tube and connects it to the base Base The bottom of the microscope, used for support

Illuminator A steady light source (110 volts) used in place of a mirror. If your microscope has a mirror, it is used to reflect light from an external light source up through the bottom of the stage. Stage The flat platform where you place your slides. Stage clips hold the slides in place. If your microscope has a mechanical stage, you will be able to move the slide around by turning two knobs. One moves it left and right, the other moves it up and down.

Revolving Nosepiece or Turret This is the part that holds two or more objective Lenses and can be rotated to easily changed power. Objective Lenses Usually you will find 3 or 4 objective lenses on a microscope. They almost always consist of 4X, 10X, 40X and 100X powers. When coupled with a 10X (most common) eyepiece lens, we get total magnifications of 40X (4X times 10X), 100X , 400X and 1000X.

How to Focus Your Microscope The proper way to focus a microscope is to start with the lowest power objective lens first and while looking from the side, crank the lens down as close to the specimen as possible without touching it. Now, look through the eyepiece lens and focus upward only until the image is sharp. If you can't get it in focus, repeat the process again. Once the image is sharp with the low power lens, you should be able to simply click in the next power lens and do minor adjustments with the focus knob. If your microscope has a fine focus adjustment, turning it a bit should be all that's necessary. Continue with subsequent objective lenses and fine focus each time.