Effect of 6-SHO on mouse prostate cancer HMVP2 cells.

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Effect of 6-SHO on mouse prostate cancer HMVP2 cells. Effect of 6-SHO on mouse prostate cancer HMVP2 cells. A, HMVP2 cells were treated with the indicated concentrations of 6-SHO for 24, 48, and 72 hours and cell survival was measured by MTT assay. The data are presented as mean±SEM. B, HMVP2 cells were treated with vehicle or 40 μmol/L 6-SHO for 48 hours and apoptosis was measured by Annexin V staining. The data are presented as mean±SEM. C, HMVP2 cells were treated with 40 μmol/L 6-SHO for 30 and 60 minutes and Western blot analysis was performed for pSTAT3Tyr705, STAT3, and β-actin (top two); HMVP2 cells were treated with 20 and 40 μmol/L 6-SHO for 2 hours followed by IL-6 for 30 minutes and Western blot analysis was performed for pSTAT3Tyr705, STAT3, and β-actin (bottom two). D, HMVP2 cells were treated with vehicle or 40 μmol/L 6-SHO followed by TNF-α treatment for 30 minutes and Western blot analysis was performed for pNF-κBp65, NF-κBp65, and β-actin (top); HMVP2 cells were treated with 20 and 40 μmol/L 6-SHO for 2 hours followed by TNF-α for 30 minutes and Western blot analysis was performed for pNF-κBp65, NF-κBp65, pIκBα, and β-actin (bottom). E, HMVP2 cells were treated with vehicle, 20 or 40 μmol/L 6-SHO for 2 hours followed by IL-6 and the level of cyclin D1 was measured by Western blot analysis. F, HMVP2 cells were treated with vehicle or 40 μmol/L 6-SHO for 2 hours followed by TNF-α and the level of cMyc was measured by Western blot analysis. Changes in relative band intensities (normalized to β-actin and total protein) for Western blot data are given at the top of each column. The results are significant (P < 0.05) where a decrease in phosphorylation or protein level is ≥40%. G, LNCaP, DU145, PC-3, and HMVP2 cells were treated with the indicated concentrations of 6-PAR, 6-GIN, or 6-SHO for 48 hours and cell survival was measured by MTT assay. The data are presented as mean±SEM. a, significantly different (P < 0.05) compared with the control group and b, compared with the other treated groups of the same compound. Achinto Saha et al. Cancer Prev Res 2014;7:627-638 ©2014 by American Association for Cancer Research