Fig. 1 Obesity-related adipocyte degeneration and cfDNA release.

Slides:

Advertisements

Similar presentations

Actin PEA Hey KOC-7c OVCA420 OVCA432 OVTOKO RMG-1 SKOV3.ip1 Supplementary Fig. S1. Expression level of endogenous PEA-15 in ovarian cancer cell.

Advertisements

Supplemental figure 1 Adipocyte SVF E CD AB Supplemental figure 1: Impaired expression of Am2 and Ramps in obese mice (A-E) mRNA levels of Am2, Crlr, Ramp1,

Venkata J. Adapala, Sunday A. Adedokun, Robert V. Considine, Kolapo M

Figure 5. Effects of spirit vinegar and natural fermented vinegar products on epidydimal triglyceride content (A), representative findings (B), and adipocyte.

Peter L. Lee, Yuefeng Tang, Huawei Li, David A. Guertin

Figure 1 Lack of endogenous estrogens increases BW and VF in mice fed normal chow or HFD. A, OVX mice (open squares) gain more weight than ovarian-intact.

Volume 19, Issue 1, Pages (January 2014)

Arterioscler Thromb Vasc Biol

Fig. 1. TP is highly expressed in myeloma.

Won Hee Choi, Jiyun Ahn, Chang Hwa Jung, Jung Sook Seo, Tae Youl Ha

Volume 17, Issue 1, Pages (January 2013)

The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.

Won Hee Choi, Jiyun Ahn, Chang Hwa Jung, Jung Sook Seo, Tae Youl Ha

Volume 8, Issue 4, Pages (October 2008)

Volume 16, Issue 10, Pages (September 2016)

Volume 21, Issue 11, Pages (December 2017)

Volume 10, Issue 5, Pages (November 2009)

Volume 15, Issue 1, Pages (January 2012)

Volume 25, Issue 2, Pages (February 2017)

Type 1 immunity drives metabolic disease but protects against NAFLD

Perturbations in the HDL metabolic pathway predispose to the development of osteoarthritis in mice following long-term exposure to western-type diet

Nida Haider, Julie Dusseault, Louise Larose iScience

Adipsin Is an Adipokine that Improves β Cell Function in Diabetes

Volume 16, Issue 7, Pages (August 2016)

Conditional deletion of MHC II on B cells does not regulate atherosclerotic plaque development in Ldlr−/− mice. Conditional deletion of MHC II on B cells.

Volume 20, Issue 13, Pages (September 2017)

Protection against High-Fat-Diet-Induced Obesity in MDM2C305F Mice Due to Reduced p53 Activity and Enhanced Energy Expenditure Shijie Liu, Tae-Hyung.

Volume 14, Issue 10, Pages (March 2016)

Volume 23, Issue 1, Pages e4 (January 2018)

Volume 17, Issue 8, Pages (November 2016)

Volume 13, Issue 8, Pages (November 2015)

Volume 13, Issue 12, Pages (December 2015)

Identification of White Adipocyte Progenitor Cells In Vivo

High-Fat Diet Triggers Inflammation-Induced Cleavage of SIRT1 in Adipose Tissue To Promote Metabolic Dysfunction Angeliki Chalkiadaki, Leonard Guarente

Volume 8, Issue 5, Pages (November 2008)

Volume 9, Issue 2, Pages (October 2014)

Volume 23, Issue 1, Pages (January 2016)

NRP1-expressing myeloid cells influence adipocyte hypertrophy, development of fatty liver, and CLSs. NRP1-expressing myeloid cells influence adipocyte.

Macrophage-resident NRP1 promotes FA uptake.

NRP1-expressing myeloid cells contribute to adipose tissue vascularization. NRP1-expressing myeloid cells contribute to adipose tissue vascularization.

Mitofusin 2 in Mature Adipocytes Controls Adiposity and Body Weight

Fig. 6 Transmissibility of adiposity from humanized mice to germ-free recipients. Transmissibility of adiposity from humanized mice to germ-free recipients.

Fig. 5 Nec-1 therapy decreases atherosclerotic lesion progression and markers of instability in Apoe−/− mice. Nec-1 therapy decreases atherosclerotic lesion.

Fig. 2 Role of TLR9 in macrophage activation.

Cellularity of epididymal adipose tissue.

Fig. 5 Hypoxic tumors from obese mice associate with increased production of IL-6 by adipocytes and myeloid cells. Hypoxic tumors from obese mice associate.

NRP1-expressing macrophages accumulate in adipose tissue during DIO and regulate weight gain and glucose tolerance. NRP1-expressing macrophages accumulate.

GLUT4 LXRE is required for downregulation of adipose GLUT4 mRNA during fasting and diet-induced obesity. GLUT4 LXRE is required for downregulation of adipose.

Skeletal muscle characteristics of HFD-induced obese mice.

TXNIP and caspase-1 protein levels are increased in the adipose tissue of ob/ob mice. TXNIP and caspase-1 protein levels are increased in the adipose tissue.

Volume 7, Issue 4, Pages (May 2014)

Volume 25, Issue 12, Pages e6 (December 2018)

PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded skin. PEDF inhibits Wnt/T-cell factor/β-catenin signaling in resting and wounded.

Effects of Rosi treatment on ASKO mice.

Fig. 1 Device structure, typical output performance, and cytocompatibility of BD-TENG. Device structure, typical output performance, and cytocompatibility.

IKKβ protects adipocytes from HFD-induced cell death.

Deficiency of adipocyte IKKβ does not affect diet-induced weigh gain but results in an exaggerated diabetic phenotype when challenged with an HFD. A: IKKβ.

a b c d e f Wankhade et al., Supplementary Figure 1

Fig. 3 Loss of SBP2 in obesity exacerbated insulin resistance and adipogenesis. Loss of SBP2 in obesity exacerbated insulin resistance and adipogenesis.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

Fig. 2 IRF8 is expressed in CD68+ macrophages after SCI.

Fig. 4 Overexpression of SBP2 improved insulin sensitivity and suppressed adipogenesis in obese mice. Overexpression of SBP2 improved insulin sensitivity.

Fig. 4 Effects of hematopoietic restoration of TLR9 on adipose tissue inflammation and insulin resistance. Effects of hematopoietic restoration of TLR9.

Fig. 3 Mmp-2−/− mice are protected from obesity and leptin resistance.

Long-range structural order control of SS-annealed cylinder patterns

Fig. 2 ITGA5 knockdown attenuates hPSC activation, differentiation, and functions. ITGA5 knockdown attenuates hPSC activation, differentiation, and functions.

Fig. 1 TLR2 expression is induced during OIS.

Fig. 2. Col IV–Ac2-26 NPs increase subendothelial collagen in Ldlr−/− mice with established atherosclerosis. Col IV–Ac2-26 NPs increase subendothelial.

Fig. 5 Effects of in vivo blockade of TLR9 on adipose tissue inflammation and insulin resistance in WT mice. Effects of in vivo blockade of TLR9 on adipose.

Fig. 3 Correction of Dmd exon 44 deletion in mice by intramuscular AAV9 delivery of gene editing components. Correction of Dmd exon 44 deletion in mice.

Presentation transcript:

Fig. 1 Obesity-related adipocyte degeneration and cfDNA release. Obesity-related adipocyte degeneration and cfDNA release. (A to C) Plasma levels of ssDNA (A), dsDNA (B), and nucleosomes (C) (n = 9). au, arbitrary units. (D and E) Levels of ssDNA (D) and dsDNA (E) in CM obtained from an organ culture experiment using epididymal fat (n = 5). (F) Correlation between plasma level of ssDNA and blood glucose level (n = 18). (G) Representative figure of Western blot analysis of perilipin expression in epididymal fat. Expression of perilipin was quantified by densitometry and normalized to the corresponding signal for β-actin (n = 5). (H) Quantitative RT-PCR analysis of TLR9 expression in epididymal fat (n = 5). (I) Cell-type–specific expression of TLR9 in epididymal fat obtained from fat-fed mice (n = 5). (J) Representative immunogold staining against ssDNA revealing accumulation of gold particles (10 nm) in the cytoplasm of macrophages (arrows) in epididymal fat obtained from fat-fed obese mice. The accumulation of gold particles was not observed in adipose tissue macrophages in lean mice (n = 4). Scale bar, 100 nm. Inset: lower magnification (scale bar, 2 μm). Cyto, cytoplasm; Nuc, nucleus. All samples were obtained from wild-type (WT) mice fed a high-fat diet (HFD) or NC for 12 weeks. *P < 0.05, **P < 0.01, and ***P < 0.001. All values are means ± SEM. Sachiko Nishimoto et al. Sci Adv 2016;2:e1501332 Copyright © 2016, The Authors