Immunoblot of scFv antibody detected with an anti-FLAG antibody.

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Immunoblot of scFv antibody detected with an anti-FLAG antibody. Immunoblot of scFv antibody detected with an anti-FLAG antibody. Inserts were grown in the expression vector pAK400. Cells were harvested and samples were extracted, separated by PAGE, and transferred to nitrocellulose membranes. They were blocked, reacted with peroxidase-coupled antibody, and visualized with a bioluminescence kit. Lane 1, uninduced with IPTG. Lane 2, blank vector. Lane 3, molecular mass marker (value indicated at the left, in kilodaltons). Lane 4, whole-cell extract from clone 10. Lane 5, periplasm extract from clone 10. Lane 6, whole-cell extract from clone 6. Lane 7, periplasm extraction from clone 6. Xiao K. Deng et al. Clin. Diagn. Lab. Immunol. 2003; doi:10.1128/CDLI.10.4.587-595.2003