Effect of bile salt on pancreatic lipase activity

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Presentation transcript:

Effect of bile salt on pancreatic lipase activity Done by :Sahar AlSaubaie

Principle:

Shake by rotation each of flasks at 15min intervals Then add 8 drops of phenolphthalein indicator to both flasks and titrate against 0.05 N sodium hydroxide to a distinctly permanent pink (30 sec at least)end point. Record the titration volumes and calculate the meg. y free fatty acids produced with and without the presence of bile salts.

Calculations: C1 X V1 = C2 X V2 Blank B: 0.01 X V1(NaOH) = C2 X 0.5 Sample A: 0.01 X V1 = C2 X 0.5 C2 (blank) – C2 (sample) =Net free fatty acid in 0.5ml of fat