Dissociation of Aβ aggregates and detection of Aβ on IME sensor

Slides:

Advertisements

Similar presentations

Fig. 2 The SOS response is required for persistence to ofloxacin but not prior treatment. The SOS response is required for persistence to ofloxacin but.

Advertisements

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Fig. 3 Comparison between predicted change in DT since the LIG and observed sea-level highstands. Comparison between predicted change in DT since the LIG.

Vibrational spectra of medieval human bones (Leopoli-Cencelle, Italy)

Fig. 5 Thermal conductivity of n-type ZrCoBi-based half-Heuslers.

Fig. 1 Evolution of magnetic field lines around a foreshock bubble in the GSE-XY plane (z = 0): Results of a hybrid simulation. Evolution of magnetic field.

Fig. 1 Map of water stress and shale plays.

Fig. 1 Examples of experimental stimuli and behavioral performance.

Fig. 3 Electron PSD in various regions.

Fig. 6 Comparison of properties of water models.

Fig. 3 Hippocampal theta oscillations during goal-directed navigation.

Fig. 2 Reference-fixing experiment, results.

Fig. 3 Glucose- and structure-dependent insulin release.

Fig. 3 Scan rate effects on the layer edge current.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Reference-fixing experiment, setup.

Fig. 3 Gate voltage dependence of the areal iDMI and PMA.

Fig. 3 FcRL1 is passively recruited into B cell immunological synapses upon BCR engagement in primary spleen B cells. FcRL1 is passively recruited into.

Fig. 1 Distribution of total and fake news shares.

Fig. 3 Characteristics of UV and temperature sensors.

Fig. 2 Expressions of cll, abd-A, and Abd-B by qRT-PCR.

Fig. 3 Photon number statistics resulting from Fock state |l, S − l〉 interference. Photon number statistics resulting from Fock state |l, S − l〉 interference.

Fig. 2 2D QWs of different propagation lengths.

Fig. 1 Impacts of intensified acidification on stream pH and calcium concentration. Impacts of intensified acidification on stream pH and calcium concentration.

Electronic structure of the oligomer (n = 8) at the UB3LYP/6-31G

Fig. 2 Variation of the SHC with carrier lifetime.

Fig. 1 Operation mechanism and scanning electron microscopy images of MTC device. Operation mechanism and scanning electron microscopy images of MTC device.

Fig. 2 EUV TG signal. EUV TG signal. Black lines in (A), (B), and (C) are the EUV TG signals from Si3N4 membranes at LTG = 110, 85, and 28 nm, respectively,

Fig. 4 EUV TG signal from Si.

Fig. 6 WPS imaging of different chemical components in living cells.

Fig. 4 DFT ωB97x/def2-TZVPP atomic charges on the sulfur atom of substituted thioaldehyde and AIMNet prediction with a different number of iterative passes.

Fig. 5 Simultaneous absence of caspase-3 and -7 is required for significant decrease of caspase-8 and -9 activation in intrinsic apoptosis. Simultaneous.

Fig. 3 Load dependence of friction force and corresponding COF.

Fig. 1 Sectional distributions of pigment concentrations measured along the Mediterranean Sea and in the Eastern Atlantic Ocean. Sectional distributions.

Fig. 3 ET dynamics on the control and treatment watersheds during the pretreatment and treatment periods. ET dynamics on the control and treatment watersheds.

Fig. 1 Histograms of the number of first messages received by men and women in each of our four cities. Histograms of the number of first messages received.

Fig. 5 Schematic phase diagrams of Ising spin systems and Mott transition systems. Schematic phase diagrams of Ising spin systems and Mott transition systems.

Fig. 4 OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with different pH. OER performance of ACoO3 (A = Ca, Sr) in alkaline solutions with.

Fig. 1 Average contribution (million metric tons) of seafood-producing sectors, 2009–2014. Average contribution (million metric tons) of seafood-producing.

Fig. 4 Evolution of fraction of sickled RBCs under hypoxia.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

Fig. 2 Schematic drawings of Göbekli Tepe skulls.

Fig. 2 NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ clean air policy. NH3, NOx, SO2, and NMVOC emission changes triggered by the JJJ.

Fig. 4 Dox-CBD-SA treatment shows reduced toxicity.

Fig. 1 Global occurrences of hydraulic fracturing–induced seismicity and potential models. Global occurrences of hydraulic fracturing–induced seismicity.

Fig. 2 Top 10 countries, ecoregions, conservation hotspots, and KBAs with the largest area of restoration hotspots. Top 10 countries, ecoregions, conservation.

Fig. 4 Relationships between light and economic parameters.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Schematic of the proposed brain-controlled assistive hearing device

Fig. 1 Distribution of forest tree mycorrhizal types and their associated factors in forests of the contiguous United States. Distribution of forest tree.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 4 Phase diagram showing significant order parameters ∣pk∣ versus T and wc. Phase diagram showing significant order parameters ∣pk∣ versus T and wc.

Multiplexed four- and eight-channel devices for rapid processing

Fig. 2 Mean field results. Mean field results. (A) Solutions P(x) to Eq. 4 for a range of T and wc = (B) Modulus ∣pk∣ of order parameters versus.

Fig. 4 The relationship between the total mean absolute momentum disturbance 〈∣p∣〉zB (in units of ℏ/D) and fringe visibility V. The relationship between.

Fig. 3 Effects of ASO and eGLP1-ASO conjugates on gene expression and protein levels in vitro in cell lines and primary mouse islet cells. Effects of ASO.

Fig. 3 Comparisons of NDVI trends over the globally vegetated areas from 1982 to Comparisons of NDVI trends over the globally vegetated areas from.

Fig. 2 Particles detected in snow samples collected at different locations from Europe to the Arctic. Particles detected in snow samples collected at different.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 4 Single-particle contact angle measurements.

Blocking of the indirect pathway impairs choice of good objects

Fig. 2 Supraballs and films from binary SPs.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Fig. 3 Rubbery strain, pressure, and temperature sensors.

Fig. 3 Calculated electronic structure of ZrCoBi.

Fig. 1 Attractive interaction between a doublon and a holon due to antiferromagnetic exchange interactions in half-filled 2D Mott insulators. Attractive.

Presentation transcript:

Fig. 2 Dissociation of Aβ aggregates and detection of Aβ on IME sensor. Dissociation of Aβ aggregates and detection of Aβ on IME sensor. (A) Scheme of Aβ monomerization by EPPS treatment after spiking plasma samples with aggregated Aβ. (B to D) Changes of Aβ levels in plasma via EPPS treatment (before and after) when Aβ aggregates were spiked into human plasma. Concentrations of Aβ are 400 pg/ml (B), 200 pg/ml (C), and 100 pg/ml (D). Statistical comparisons were made with two-tailed t test: P = 0.0003 (B) and P = 0.0146 (D). Black squares, untreated samples; white squares, EPPS-treated samples. (E) IME chip and the enlarged image of one IME pair (scanning electron microscopic image). (F) Sequential process for surface modification of the IME sensor. The IME sensor was sequentially functionalized with APMES, PVP-CHO, glutaraldehyde, Aβ antibody (6E10), and BSA. (G) Image of IME sensor and PDMS microfluidic chip. Each sample was injected into two microfluidic channels on the IME unit chip. (H) IME unit chip with PDMS microfluidic chip photograph. Two channels were filled with colored solutions. (I) Impedance change (|ΔZ/Z0|, %) of the IME by interaction between Aβ and its antibody. (J) Logarithmical linear sensitivity to Aβ levels (n = 5). Error bars indicate SDs. (K and L) Analysis of mouse plasma Aβ levels [black dot, WT (n = 9); red dot, TG (n = 9)]. (K) WT mouse plasma without and with EPPS treatment. (L) TG mouse plasma without and with EPPS treatment. The dot data represent multiple (n = 5) independent experiments. Two-tailed t tests were performed in the statistical analyses (*P < 0.05 and ***P < 0.001; nonsignificant analysis is not indicated). YoungSoo Kim et al. Sci Adv 2019;5:eaav1388 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).