Batch variation of formulations from two products by two different genomic-scale techniques. Batch variation of formulations from two products by two different.

Slides:

Advertisements

Similar presentations

Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University.

Advertisements

EQTLs.

Functional Genomics in Evolutionary Research

Overview Expression data basics Introduction Biological network data

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

Exploration of the data set with age as a continuous variable.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

HIS-24 regulates expression of infection-inducible genes.

Genomic alterations in breast cancer cell line MDA-MB-231.

Relative abundances of the top 10 most abundant OTUs across all fish samples are shown along with the data from the probiotic B. pumilus RI06-95 (OTU 3673)

Transdomain taxonomic profiles from subway shotgun metagenomes.

Genomic rearrangements of E

MFS1 expression in Z. tritici field isolates with different promoter genotypes. MFS1 expression in Z. tritici field isolates with different promoter genotypes.

Genomic context, sequence identity, and structural homology modeling of the aadA31 gene detected in P. multocida PM13 and H. somni HS31. Genomic context,

Fold change differences for each bacterial genus in the two fraction pairs. Fold change differences for each bacterial genus in the two fraction pairs.

Size profile of viral small RNAs obtained from control cells and RIG-I- and MDA5-deficient (KO) cells after infection for 24 h with SINV at an MOI of 0.1,

Isolation and phylogenetic characterization of ISS strains.

Positions of LSUCC isolates relative to matching OTUs within the top 60 ranks for the most successful experiment, CJ2, which is enlarged to allow for OTU.

Differential relative abundance of major taxa for successive pairwise comparisons. Differential relative abundance of major taxa for successive pairwise.

Whole-cell patch-clamp analysis of NAc cells.

Phylogenetic tree of 170 Lactobacillus species.

Comparison of gene expression maps from available S

(A and B) Relative (A) and mean (B) abundances of reference viral genomes across the continuum summarized by host type. (A and B) Relative (A) and mean.

Comparative analysis of Aeromonas sp.

Phylogenetic diversity of archaeal lineages in the great ape gut microbiome. Phylogenetic diversity of archaeal lineages in the great ape gut microbiome.

High-density foci of Giardia colonization are present in the proximal small intestine of mice and gerbils. High-density foci of Giardia colonization are.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

TIVAP catheter collection from patients suspected or not of catheter-related infection from two French hospitals. TIVAP catheter collection from patients.

Whole-genome comparison of E

(a, b) Hypothetical scenario where 2 samples of 2 proportions may explain two different scenarios in the environment. (a, b) Hypothetical scenario where.

Relative ITS2 abundance of Saccharomyces in stool over time as a Saccharomyces cerevisiae-free diet was consumed. Relative ITS2 abundance of Saccharomyces.

Relative abundances of Propionibacterium species in different skin areas determined by 16S rRNA gene sequence analysis of 10 individuals. Relative abundances.

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

Spearman correlation of normalized relative abundances between MAGs from TBE6 and taxa in the epilimnion of the environment of origin (Trout Bog Lake).

Framework for integrating taxonomic and metabolomic data.

Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance.

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

Phylogenetic and convergence analyses of rpoB mutations.

(A) Taxonomic identity at the phylum level of raw leachate (RL) and enrichment microcosms (E) as determined via Ion Torrent 16S rRNA gene amplicon sequencing.

Relative abundances of microeukaryotic taxa detected by ITS2 analysis of DNA or cDNA from stool. Relative abundances of microeukaryotic taxa detected by.

Relationship between relative abundance and transcription/abundance ratio (logarithmic scales) of OTUs (brown), ZOTUs (turquoise), and population genomes.

Nonmetric multidimensional scaling (NMDS) (A) and dendrogram and heat map (B) of the relative abundances of the data in the custom database across the.

Dynamic light scattering (DLS) analysis of S

The Amazon viral scaffolds and viral genomes most important for river and plume segregation. The Amazon viral scaffolds and viral genomes most important.

Whole-chromosome view of gene density, inverse environmental variation, inverse total variation, and open chromatin signature. Whole-chromosome view of.

Impact of medical data on bacterial diversity of fractionated samples.

Sequence variation of 16S rRNA gene primer-binding sites.

OTU and family-level changes in murine gut as a result of acarbose feeding on a high-starch diet. OTU and family-level changes in murine gut as a result.

KEY CONCEPT Entire genomes are sequenced, studied, and compared.

Bacterial composition of olive fermentations is affected by microbial inoculation. Bacterial composition of olive fermentations is affected by microbial.

Enrichment of KEGG pathways in microbial genes in different samples.

Functional microbiome differences between high-fat and standard chow diets. Functional microbiome differences between high-fat and standard chow diets.

ITS rRNA gene locus. ITS rRNA gene locus. Schematic of the eukaryotic ribosomal gene cluster. The SILVA database contains sequences of the 18S gene, while.

Sequence comparisons of fungal HOG1 orthologues.

Identification of a ufo1 Candidate Gene from the Mapping Region.

Distribution of the diversity numbers of antibiotic resistance and virulence factor protein families by environmental metagenomes’ protein family richness.

Comparison between early-life and year 2 AMR levels.

RNA-seq analysis from the MVI-it culture indicates that a majority of the BGHV8 coding sequences are transcribed. RNA-seq analysis from the MVI-it culture.

LEfSe comparison analysis between the control and ciprofloxacin or vancomycin-imipenem groups at the end of antibiotic treatment (A or B, respectively)

Normalized abundance of tetracycline resistance genes in Swedish infants by AMR++. Normalized abundance of tetracycline resistance genes in Swedish infants.

Phylogeny and expression landscape of Wolbachia in D. melanogaster.

Cell lines with aberrant expression of NRG1 are exquisitely sensitive to downregulation of ERBB3 signaling. Cell lines with aberrant expression of NRG1.

Locations of the Riverton City dump and adjacent Duhaney River sampling sites. Locations of the Riverton City dump and adjacent Duhaney River sampling.

Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock communities. Relative abundance of taxa in the 16S rRNA PCR amplicon and gDNA mock.

Variations in beta and alpha diversity of gut microbiome eukaryotic communities explained by presence of Blastocystis. Variations in beta and alpha diversity.

Total ARG reads normalized by read length and 16S gene read count in early life in Bangladesh. Total ARG reads normalized by read length and 16S gene read.

Integrated analysis of gene expression and copy number alterations.

Comparison of 16S sequencing and shallow shotgun recovery of species-level taxa. Comparison of 16S sequencing and shallow shotgun recovery of species-level.

Fig. 3 Postnatal assembly of the humanized gut microbiota.

Presentation transcript:

Batch variation of formulations from two products by two different genomic-scale techniques. Batch variation of formulations from two products by two different genomic-scale techniques. (A) The relative abundance of each bacterium in a product as determined by k-mer analysis of metagenomic sequencing. The results are for two examples, products G and H, across three respective lots. (B) Analysis of the same samples with the custom-designed high-density GutProbe microarray platform (9). Each spot is a summarized probe set intensity (red shading, <8.5 absent; blue shading, ≥8.5 present) for a specific gene array from available whole genomes of human gut-associated species, including common probiotics. As has been shown previously, this genomic-scale genotyping is exquisitely sensitive for the determination of the identities and formulations of products, especially when they are assessed in pairwise comparisons. Jennifer N. Patro et al. mSphere 2016; doi:10.1128/mSphere.00057-16