Overexpression of sRNA-29 changes the expression of 53 genes across the P. denitrificans genome. Overexpression of sRNA-29 changes the expression of 53.

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Overexpression of sRNA-29 changes the expression of 53 genes across the P. denitrificans genome. Overexpression of sRNA-29 changes the expression of 53 genes across the P. denitrificans genome. (A) Volcano plot showing upregulated (red) and downregulated (blue) transcripts (P < 0.05) from RNA-seq data under conditions of overexpression of sRNA-29 in the presence of taurine (n = 3) versus the empty vector in the presence of taurine (n = 3). The dashed horizontal line represents an adjusted P value of 0.05. (B and C) qRT-PCR validation of changes in expression of genes of interest under conditions of sRNA-29 plus empty vector (B) or under conditions of high levels of NO2 emission. Hannah Gaimster et al. mBio 2019; doi:10.1128/mBio.01165-19