Naomi Balaban, Yael Gov, Arkady Bitler, Johan R. Boelaert

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Prevention of Staphylococcus aureus biofilm on dialysis catheters and adherence to human cells Naomi Balaban, Yael Gov, Arkady Bitler, Johan R. Boelaert Kidney International Volume 63, Issue 1, Pages 340-345 (January 2003) DOI: 10.1046/j.1523-1755.2003.00733.x Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 1 RNAIII inhibiting peptide (RIP) reduces adherence ofS. aureus to HaCat cells. (A) FITC-labeled bacterial cells were applied to confluent HaCat cells with/without RIP. Adherent fluorescent bacteria were measured at 485/530nm. *P < 0.05. (B) F actin was labeled with Rh-phalloidin and cells analyzed by confocal scanning laser microscopy. Bacteria appear as light dots and actin filaments as light lines. Bar = 20 μm. Kidney International 2003 63, 340-345DOI: (10.1046/j.1523-1755.2003.00733.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 2 RIP reduces adherence ofS. aureus to polystyrene. The atomic force microscopy study used a larger scale to observe number of adherent bacteria in a biofilm (A, B) and a smaller scale to observe adherence of a single bacterium (C, D). S. aureus clinical strain 1 were applied to polystyrene cell culture plates with RIP (B and D) or without RIP (A and C) and cells observed using atomic force microscopy in tapping mode. Amplitude images were collected and areas of cell attachment were compared. The direction of scan is from left to right. The color code of amplitude images corresponds to the changes in the amplitude of tapping. Top bar (relevant for A and B) corresponds to 2.5 μM. Bottom bar (relevant for C and D) corresponds to 0.5 μM. Kidney International 2003 63, 340-345DOI: (10.1046/j.1523-1755.2003.00733.x) Copyright © 2003 International Society of Nephrology Terms and Conditions

Figure 3 RIP reduces adherence ofS. aureus to silicone (A) and to polyurethane (B). Cells (S. aureus RN6911, strain 1 and strain 20) were incubated with silicone- or polyurethane-made dialysis catheter fragments in the presence (□) or absence (▪) of RIP. Adherent bacteria were stained with safranin and absorbance was determined at 490nm. Kidney International 2003 63, 340-345DOI: (10.1046/j.1523-1755.2003.00733.x) Copyright © 2003 International Society of Nephrology Terms and Conditions