Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. A, Treatment.

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Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. Treatment with octyl-D-2-HG and octyl-L-2-HG reduces MIR148A expression. A, Treatment with octyl-D-2-HG (1.0 mmol/L) significantly reduces MIR148A expression in 293T cells. Transcript levels in cells treated by vehicle and octyl-α-KG determined at passage 34-36 by RT-qPCR (n = 2, bars show SEM). B, Treatment with octyl-D-2-HG and octyl-L-2-HG significantly reduces MIR148A expression in NHA cells. Transcript levels in cells treated by vehicle, octyl-α-KG, -D-2-HG and -L-2-HG determined by RT-PCR (inset above, n = 2, combined on single gel) and RT-qPCR (below; n = 3, bars show SEM). RUN6B transcription was used as an internal control for RT-PCR. Statistical analysis in all graphs performed using two-tailed Student t test. ***, P < 0.001; **, P < 0.01; *, P < 0.05 compared with control. Tie Li et al. Mol Cancer Res 2018;16:947-960 ©2018 by American Association for Cancer Research