Purified LF is cytotoxic to WEHI 164 cells.

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Purified LF is cytotoxic to WEHI 164 cells. Purified LF is cytotoxic to WEHI 164 cells. (A) Octyl-Sepharose chromatogram of T. vaginalis extract with LF elution at 30% N-propanol. (B) Cytotoxicity of LF and bovine serum albumin (BSA), purified by octyl-Sepharose chromatography as described in the text, lyophilized, and reconstituted in Hanks' balanced salt solution. Cytotoxicity was measured on WEHI 164 cells as described previously. Data shown are means and standard deviations for three individual experiments. *, significant difference (P < 0.05) between treatment groups. Jane R. Schwebke, and Donald Burgess Clin. Microbiol. Rev. 2004; doi:10.1128/CMR.17.4.794-803.2004