In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo,

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Presentation transcript:

In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection Sílvia Mateo, M.Sc., Mònica Parriego, M.Sc., Montserrat Boada, Ph.D., Francesca Vidal, Ph.D., Buenaventura Coroleu, M.D., Anna Veiga, Ph.D. Fertility and Sterility Volume 99, Issue 3, Pages 897-902.e1 (March 2013) DOI: 10.1016/j.fertnstert.2012.11.014 Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 1 (A) Monopronuclear (1PN) zygote, 16 to 20 hours after ICSI. (B) Blastocyst image from a 1PN ICSI zygote (D+5). (C) Fixation of 5th day blastocyst (magnification: ×10). In the smaller image, nuclei detail (magnification: ×20). (D) Same image processed by FISH showing to XY diploid constitution: two chromosomes 13 (red), two 18 (aqua), two 21 (green), one X (blue), and one Y (gold). Fertility and Sterility 2013 99, 897-902.e1DOI: (10.1016/j.fertnstert.2012.11.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions

Figure 2 Embryo classification according to chromosome constitution. (adapted from Delhanty et al., 1997 [9]). Fertility and Sterility 2013 99, 897-902.e1DOI: (10.1016/j.fertnstert.2012.11.014) Copyright © 2013 American Society for Reproductive Medicine Terms and Conditions