Fig. 2 IDH3α regulates GBM progression in vivo.

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Fig. 2 IDH3α regulates GBM progression in vivo. IDH3α regulates GBM progression in vivo. (A to G) IDH3α ablation reduces glioma tumor progression. (A) Western blot (WB) showing IDH3α expression in CRISPR-Cas9–modified NHAs; NHAs were transduced with a scrambled single-guide RNA (sgRNA) (Ctrl) and two different IDH3α-targeting sgRNAs, i.e., sgRNA-318 and sgRNA-329. Hsp70 is shown as a control. (B) In vitro cell growth curves of IDH3α WT and IDH3α KO NHAs. Cells were counted every 3 days using trypan blue and an automated cell counter. Means ± SD are shown; n = 3; *P < 0.05, **P < 0.005. (C) Representative in vivo imaging system (IVIS) images of brain tumor–bearing mice, demonstrating reduced bioluminescence in IDH3α KO tumor–bearing mice compared to the WT control. (D) Quantification of bioluminescence signal at day 13 after intracranial cell inoculation. Means ± SEM are shown; *P < 0.05, ***P < 0.0005; n = 10 per group. (E) Kaplan-Meier survival curves of mice orthotopically implanted with IDH3α WT or KO NHAs. *P < 0.05, ***P < 0.0005; n = 10 per group. (F) Representative H&E stainings of tumor sections from IDH3α WT and KO tumors. (G) Number of mitoses in tumors per high-power field (hpf): two tumors per group and eight slices per tumor. **P < 0.005, one-way ANOVA. (H to K) RNA interference (RNAi)–mediated IDH3α knockdown (KD) reduces tumor progression in PDX mice. (H) WB analysis for IDH3α in different GIC clones modified for stable IDH3α KD. Hsp70 is shown as a loading control. (I) Representative IVIS images of PDX-bearing mice. (J) Quantification of bioluminescence signal 40 and 47 days after tumor implantation. *P < 0.05; n = 8 per group. (K) Kaplan-Meier survival curves of mice intracranially injected with GICs modified for IDH3α stable KD in comparison to PDX tumors expressing a scrambled control shRNA. *P < 0.05; n = 8 per group. (L to O) IDH3α overexpression in GICs promotes GBM tumor progression. (L) WB analysis for endogenous and FLAG epitope–tagged IDH3α. Hsp70 is shown as a loading control. (M) Representative IVIS images of mice harboring vector (CSII) control and IDH3αFLAG-expressing brain tumors. (N) Quantification of bioluminescence signal in mice 35 and 42 days after cell inoculation. *P < 0.05; n = 10 per group. (O) Kaplan-Meier survival curves of mice intracranially injected with CSII vector control and IDH3α-overexpressing GICs. ***P < 0.0005; n = 10 per group. Jasmine L. May et al. Sci Adv 2019;5:eaat0456 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).