Leanne Pacella, Deirdre L. Zander-Fox, Ph. D. , David T. Armstrong, Ph

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Presentation transcript:

Women with reduced ovarian reserve or advanced maternal age have an altered follicular environment Leanne Pacella, Deirdre L. Zander-Fox, Ph.D., David T. Armstrong, Ph.D., Michelle Lane, Ph.D. Fertility and Sterility Volume 98, Issue 4, Pages 986-994.e2 (October 2012) DOI: 10.1016/j.fertnstert.2012.06.025 Copyright © 2012 Terms and Conditions

Figure 1 Comparison of granulosa cell (GC) and cumulus cell (CC) glucose uptake (A), lactate production (B), glycolytic index of GCs and CCs (C) and gene expression of phosphofructokinase platelet (PFKP) (D) and lactate dehydrogenase A (LDHA) (E). (A–C) n = 4 patients per treatment group; (D, E) n = 5 patients per treatment group. (A, B) a–c: significant differences between treatment groups for cell type (P<.05). (C) a–f: significant differences between treatment groups. (D, E) a–c: significant differences between treatment groups. AMA = advanced maternal age; NOR = normal ovarian reserve; ROR = reduced ovarian reserve; YMA = young maternal age. Fertility and Sterility 2012 98, 986-994.e2DOI: (10.1016/j.fertnstert.2012.06.025) Copyright © 2012 Terms and Conditions

Figure 2 Progesterone production by cultured granulosa cells (GCs) (A) and cumulus cells (CCs) (B). Gene expression of the P4 receptor (PGER) (C), prostaglandin E receptor-2 (PTGER2) (D), cytosolic phospholipase A2 (cPLA2) (E), and tumor protein 53 (p53) (F) in CCs. (A, B) n = 6 patients per treatment group; (C–F) n = 3 patients per treatment group. (A, B) a–c: significant differences between treatment groups (P<.05). (C–F) a–c: significant differences between treatment groups (P<.05). AMA = advanced maternal age; NOR = normal ovarian reserve; ROR = reduced ovarian reserve; YMA = young maternal age. Fertility and Sterility 2012 98, 986-994.e2DOI: (10.1016/j.fertnstert.2012.06.025) Copyright © 2012 Terms and Conditions

Supplemental Figure 1 Random allocation of patients to each experimental protocol. *Six patients were randomly allocated to both the follicular fluid (FF) analysis and P4 production experimental protocols; ‡ 9 patients were randomly allocated to both the P4 production and gene expression experimental protocols; † 15 patients were randomly allocated to both the FF and glycolytic enzyme expression experimental protocols. Fertility and Sterility 2012 98, 986-994.e2DOI: (10.1016/j.fertnstert.2012.06.025) Copyright © 2012 Terms and Conditions