Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling Christian F.S. Jensen, B.Sc., Dana A. Ohl, M.D.,

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Optimizing human semen cryopreservation by reducing test vial volume and repetitive test vial sampling Christian F.S. Jensen, B.Sc., Dana A. Ohl, M.D., Walter R. Parker, M.D., Andre M. da Rocha, Ph.D., Laura M. Keller, A.A.S., Timothy G. Schuster, M.D., Jens Sonksen, M.D., Ph.D., Gary D. Smith, Ph.D. Fertility and Sterility Volume 103, Issue 3, Pages 640-646.e1 (March 2015) DOI: 10.1016/j.fertnstert.2014.12.107 Copyright © 2015 Terms and Conditions

Figure 1 Test vial mean ± SEM cryosurvival at multiple volumes. a,b,cDifferent letters are significantly different (P<.05). Fertility and Sterility 2015 103, 640-646.e1DOI: (10.1016/j.fertnstert.2014.12.107) Copyright © 2015 Terms and Conditions

Figure 2 Long-term evaluation (mean ± SEM). (A) Post-thaw motility, (B) progressive motility, and (C) cryosurvival of samples stored in VN2 for donor 1 (2004–2008) and donor 2 (2009–2011). Fertility and Sterility 2015 103, 640-646.e1DOI: (10.1016/j.fertnstert.2014.12.107) Copyright © 2015 Terms and Conditions

Supplemental Figure 1 Long-term evaluation. (A) Recording of the temperature at the top and middle of the VN2 tank. (B) Monthly recording of liquid nitrogen height and usage at the bottom of the VN2 tank. Fertility and Sterility 2015 103, 640-646.e1DOI: (10.1016/j.fertnstert.2014.12.107) Copyright © 2015 Terms and Conditions