Fig. 1 X-ray structures of the ScoI·Cu2+ and CoxB·CuA holo-proteins, and formation of the ScoI·Cu2+·CoxB complex. X-ray structures of the ScoI·Cu2+ and.

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Fig. 1 X-ray structures of the ScoI·Cu2+ and CoxB·CuA holo-proteins, and formation of the ScoI·Cu2+·CoxB complex. X-ray structures of the ScoI·Cu2+ and CoxB·CuAholo-proteins, and formation of the ScoI·Cu2+·CoxB complex. (A and B) Cartoon representations of the 1.4-Å structure of the periplasmic ScoI domain (18.1 kDa) with bound Cu2+ (A) and the 1.3-Å structure of the periplasmic CoxB domain (15.6 kDa) harboring the CuA center (B). Top, overall folds; bottom, coordination spheres around the bound copper ions (orange). Residues coordinating Cu are shown as stick models, and distances between the liganding atoms and the Cu ions are given. (C) Cw EPR spectra of ScoI·Cu2+ (red) and the ScoI·Cu2+·CoxB complex (dark gray), normalized to identical concentration. (D) Cw EPR spectrum of CoxB·CuA. (E) Analysis of ScoI·Cu2+·CoxB complex (CP) formation at pH 7.0 and 25°C by gel filtration. CoxB (40 μM) was titrated with the indicated amounts of ScoI·Cu2+. (F) Titration of 15 μM CoxB with ScoI·Cu2+ at pH 7.0 and 25°C, recorded via the ScoI·Cu2+·CoxB–specific absorption at 520 nm. The sharp kink at the 1:1 ratio indicates that ScoI·Cu2+·CoxB is a high-affinity complex with a dissociation constant below 10−7 M. (G) Stopped-flow absorbance kinetics of ScoI·Cu2+·CoxB complex formation at 25°C and pH 7.0, recorded at 520 nm. CoxB (25 μM) was mixed with a 1.5-, 3-, or 5-fold excess of ScoI·Cu2+. Absorbance traces (dotted lines) were fitted globally (solid lines) to a mechanism assuming formation of an encounter complex (association: k1 = 1.5 ± 0.014 × 105 M−1 s−1; dissociation: k2 = 1.68 ± 0.04 s−1) followed by intramolecular rearrangement to the final complex (k3 = 1.20 ± 0.01 s−1). Fabia Canonica et al. Sci Adv 2019;5:eaaw8478 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).