Induction of Mixed Chimerism through Transplantation of CD45-Congenic Mobilized Peripheral Blood Stem Cells after Nonmyeloablative Irradiation Zvonimir.

Slides:

Advertisements

Similar presentations

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

Advertisements

Erik Ames, Salif Harouna, Colin Meyer, Lisbeth A. Welniak, William J

Dipeptidyl peptidase IV (DPPIV/CD26) inhibition does not improve engraftment of unfractionated syngeneic or allogeneic bone marrow after nonmyeloablative.

Expression of Chemokines in GVHD Target Organs Is Influenced by Conditioning and Genetic Factors and Amplified by GVHR Markus Y. Mapara, Corinna Leng,

Michael A. Pulsipher, Christina Peters, Ching-Hon Pui

How to Treat MDS without Stem Cell Transplantation

Induction of Immunity to Neuroblastoma Early after Syngeneic Hematopoietic Stem Cell Transplantation Using a Novel Mouse Tumor Vaccine Weiqing Jing,

Apoptotic Donor Leukocytes Limit Mixed-Chimerism Induced by CD40-CD154 Blockade in Allogeneic Bone Marrow Transplantation Jian-ming Li, John Gorechlad,

Vaccination of Children following Allogeneic Stem Cell Transplantation

Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J

A Non-Leaky Artemis-Deficient Mouse That Accurately Models the Human Severe Combined Immune Deficiency Phenotype, Including Resistance to Hematopoietic.

Human CD4+CD25+ Cells in Combination with CD34+ Cells and Thymoglobulin to Prevent Anti-hematopoietic Stem Cell T Cell Alloreactivity Dolores Mahmud,

Low c-Kit Expression Level Induced by Stem Cell Factor Does Not Compromise Transplantation of Hematopoietic Stem Cells Chia-Ling Chen, Katerina Faltusova,

FLT3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides.

Pharmacologic Expansion of Donor-Derived, Naturally Occurring CD4+Foxp3+ Regulatory T Cells Reduces Acute Graft-versus-Host Disease Lethality Without.

Jacob Andrade, Shundi Ge, Goar Symbatyan, Michael S. Rosol, Arthur J

Volume 3, Issue 5, Pages (November 2014)

FTY720 Markedly Increases Alloengraftment but Does Not Eliminate Host Anti-Donor T Cells that Cause Graft Rejection on Its Withdrawal Patricia A. Taylor,

Characterizing Donor-Derived Cells in Nonhematopoietic Tissue

Sabina Kersting, Leo F. Verdonck

Dipeptidyl peptidase IV (DPPIV/CD26) inhibition does not improve engraftment of unfractionated syngeneic or allogeneic bone marrow after nonmyeloablative.

Activated Allogeneic NK Cells as Suppressors of Alloreactive Responses

Survival and Function of MiHA Epitope-Specific Host CD8 TM Cells Following Ablative Conditioning and HCT Alwi M. Shatry, Derry C. Roopenian, Robert B.

Let Them Eat! Comparing a Neutropenic Diet to a Food Safety Based Diet in Patients Undergoing Hematopoietic Stem Cell Transplantation (HSCT) Cynthia.

Hydrodynamic Delivery of Human IL-15 cDNA Increases Murine Natural Killer Cell Recovery after Syngeneic Bone Marrow Transplantation Isabel Barao, Maite.

Volume 4, Issue 2, Pages (February 2003)

Donor-to-Recipient ABO Mismatch Does Not Impact Outcomes of Allogeneic Hematopoietic Cell Transplantation Regardless of Graft Source Sharat Damodar,

J. J. Nietfeld, Marcelo C. Pasquini, Brent R

Christopher C. Dvorak, Rajni Agarwal, Gary V. Dahl, John J

HCMV Infection of Humanized Mice after Transplantation of G-CSF–Mobilized Peripheral Blood Stem Cells from HCMV-Seropositive Donors Morgan Hakki, Devorah.

A Process to Facilitate Decision Making in Pediatric Stem Cell Transplantation: The Individualized Care Planning and Coordination Model Justin N. Baker,

Lymphocyte Subset Recovery and Outcome after Autologous Hematopoietic Stem Cell Transplantation for Plasma Cell Myeloma Jessica Rueff, Michael Medinger,

T Cell and B Cell Immunity can be Reconstituted with Mismatched Hematopoietic Stem Cell Transplantation Without Alkylator Therapy in Artemis-Deficient.

A CD4 Domain 1 CC′ Loop Peptide Analogue Enhances Engraftment in a Murine Model of Bone Marrow Transplantation with Sublethal Conditioning Gabor Varadi,

Correlation between the numbers of naive T cells infused with blood stem cell allografts and the counts of naive T cells after transplantation Jan Storek,

Donor Selection in T Cell–Replete Haploidentical Hematopoietic Stem Cell Transplantation: Knowns, Unknowns, and Controversies Stefan O. Ciurea, Richard.

Effect of Total Nucleated and CD34+ Cell Dose on Outcome after Allogeneic Hematopoietic Stem Cell Transplantation Mats Remberger, Johan Törlén, Olle.

Nasal Epithelial Cells of Donor Origin after Allogeneic Hematopoietic Cell Transplantation are Generated at a Faster Rate in the First 3 Months Compared.

High White Blood Cell Concentration in the Peripheral Blood Stem Cell Product Can Induce Seizures during Infusion of Autologous Peripheral Blood Stem.

Joseph Pidala, Stephanie J

Pharmacokinetic Evaluation of the Drug Interaction between Intravenous Itraconazole and Intravenous Tacrolimus or Intravenous Cyclosporin A in Allogeneic.

Eva Guinan, Leo Luzmik, Rupert Handgretinger, Ann Woolfrey

FLT3L and Plerixafor Combination Increases Hematopoietic Stem Cell Mobilization and Leads to Improved Transplantation Outcome Shun He, Jianhong Chu,

Disparity in Survival Outcome after Hematopoietic Stem Cell Transplantation for Hematologic Malignancies According to Area of Primary Residence Keshav.

Shigeo Fuji, Hermann Einsele, Bipin N. Savani, Markus Kapp

Central Nervous System Involvement in Acute Myeloid Leukemia Patients Undergoing Hematopoietic Cell Transplantation Merav Bar, Weigang Tong, Megan Othus,

Cytokines and cytotoxic pathways in engraftment resistance to purified allogeneic hematopoietic stem cells Christian Scheffold, Yolanda C. Scheffold,

Volume 4, Issue 2, Pages (February 2009)

Clinical Endpoints in Allogeneic Hematopoietic Stem Cell Transplantation Studies: The Cost of Freedom Haesook T. Kim, Philippe Armand Biology of Blood.

Adverse Prognostic Factors for Morbidity and Mortality During Peripheral Blood Stem Cell Mobilization in Patients with Light Chain Amyloidosis Jason.

Brile Chung, Eric Dudl, Akira Toyama, Lora Barsky, Kenneth I. Weinberg

Regression Models for Hazard Rates Versus Cumulative Incidence Probabilities in Hematopoietic Cell Transplantation Data Brent R. Logan, Mei-Jie Zhang,

Pauline Damien, David S. Allan

Lack of correlation between an assay used to determine early marrow allograft rejection and long-term chimerism after murine allogeneic bone marrow transplantation:

Galactomannan Antigen Enzyme-Linked Immunosorbent Assay for Diagnosis of Invasive Aspergillosis after Hematopoietic Stem Cell Transplantation Patrick.

What is quality in a transplant program?

High Incidence of Radiation-Induced Cavernous Hemangioma in Long-Term Survivors Who Underwent Hematopoietic Stem Cell Transplantation with Radiation Therapy.

Chimeric Antigen Receptor–Modified T Cells: Clinical Translation in Stem Cell Transplantation and Beyond Stanley R. Riddell, Michael C. Jensen, Carl.

Blood and Marrow Transplant Handbook

Chimerism Patterns of Long-Term Stable Mixed Chimeras Posthematopoietic Stem Cell Transplantation in Patients with Nonmalignant Diseases: Follow-Up of.

Low Serum Levels of Total Rabbit-IgG Is Associated with Acute Graft-Versus-Host Disease after Unrelated Donor Hematopoietic Stem Cell Transplantation:

Pharmacological Immunosuppression Reduces But Does Not Eliminate the Need for Total-Body Irradiation in Nonmyeloablative Conditioning Regimens for Hematopoietic.

All Hematopoietic Stem Cells Engraft in Submyeloablatively Irradiated Mice Katarina Forgacova, Filipp Savvulidi, Ludek Sefc, Jana Linhartova, Emanuel.

Addition of treosulfan to a nonmyeloablative conditioning regimen results in enhanced chimerism and immunologic tolerance in an experimental allogeneic.

Mammen Chandy Biology of Blood and Marrow Transplantation

Yuri Fedoriw, T. Danielle Samulski, Allison M. Deal, Cherie H

Addition of Plerixafor to Mobilization Regimens in Autologous Peripheral Blood Stem Cell Transplants Does Not Affect the Correlation of Preharvest Hematopoietic.

What Role Is There for Antithymocyte Globulin in Allogeneic Nonmyeloablative Canine Hematopoietic Cell Transplantation? Razvan Diaconescu, Marie-Térèse.

Mary Eapen Biology of Blood and Marrow Transplantation

Optimal Donor Selection: Beyond HLA

Presentation transcript:

Induction of Mixed Chimerism through Transplantation of CD45-Congenic Mobilized Peripheral Blood Stem Cells after Nonmyeloablative Irradiation Zvonimir Koporc, Sinda Bigenzahn, Peter Blaha, Elahi Fariborz, Edgar Selzer, Megan Sykes, Ferdinand Muehlbacher, Thomas Wekerle Biology of Blood and Marrow Transplantation Volume 12, Issue 3, Pages 284-292 (March 2006) DOI: 10.1016/j.bbmt.2005.11.011 Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 1 Transplantation of PBSCs leads to significantly lower levels of long-term chimerism in comparison with BMT after nonmyeloablative TBI. CD45.1 mice (n = 6/group) received either 20 × 106 CD45.2 BMC (A, B, C) or 20 × 106 CD45.2 mPBSCs (D, E, F) after various doses of TBI (2 Gy [A,D], 1.5 Gy [B, E], or 1 Gy [C, F). The percentages of donor-derived CD4+ cells, CD8+ cells, B cells, monocytes/granulocytes, and NK cells among white blood cells were evaluated by FCM at different time points and are shown as means. Levels of chimerism induced with mPBSCs were significantly lower than those induced with BMCs treated with the same TBI dose (A vs D, B vs E, and C vs F; P < .05). HSCT denotes hematopoietic stem cell transplantation. Biology of Blood and Marrow Transplantation 2006 12, 284-292DOI: (10.1016/j.bbmt.2005.11.011) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 2 Transplantation of similar numbers of c-kit+ mPBSCs and c-kit+ BMCs still led to differences in chimerism levels. CD45.1 mice (n = 6/group) received either 10 × 106 CD45.2 BMCs (A, C) or 30 × 106 CD45.2 mPBSCs (B, D) with 1.5 Gy of TBI. In this manner, similar numbers of c-kit+ cells were transplanted in both groups (according to the percentage of c-kit+ cells as determined by FCM analysis among unseparated mPBSCs and BMCs). To evaluate whether the lower chimerism induced in mPBSCT recipients is due to a remaining alloresponse against CD45.2, or to other antigenic differences, some groups received immunosuppression (C, D) (rapa, MP, and MMF for four weeks), others remained untreated (A, B). Chimerism levels in various lineages as determined by FCM analysis over time are shown as means. *P < .05 indicates a significant difference in chimerism between the BMT and mPBSC transplantation groups (A vs B and C vs D). IS treatment did not significantly improve levels of chimerism (P = NS in any lineage) by either BMT or mPBSC transplantation (A vs C or B vs D). HSCT denotes hematopoietic stem cell transplantation. Biology of Blood and Marrow Transplantation 2006 12, 284-292DOI: (10.1016/j.bbmt.2005.11.011) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions

Figure 3 Levels of chimerism induced in BMC and mPBSC recipients correlated with the number of transplanted progenitor cells as estimated from CFU assays. Chimerism per transplanted progenitor cell was calculated as described in detail in the Methods section. A significant difference in percentage of chimerism per 1 × 104 transplanted progenitor cells, between BMC and mPBSC recipients (A vs B) is noticeable only in the first few weeks after HSCT (C), probably due to the larger percentage of CD4+ and CD8+ cells contained in mPBSC. Data are from the same experiment for which results are also shown in Figure 3. HSCT denotes hematopoietic stem cell transplantation. Biology of Blood and Marrow Transplantation 2006 12, 284-292DOI: (10.1016/j.bbmt.2005.11.011) Copyright © 2006 American Society for Blood and Marrow Transplantation Terms and Conditions