Tissue proteomics: a new investigative tool for renal biopsy analysis

Slides:

Advertisements

Similar presentations

The Case | Renal failure in a bodybuilder athlete

Advertisements

Renal manifestations in Fabry disease and therapeutic options

The Case | Diabetic nephropathy in a nondiabetic smoker?

Stem cells for kidney repair: useful tool for acute renal failure?

Volume 75, Issue 9, Pages (May 2009)

C3 deposition glomerulopathy due to a functional Factor H defect

Prehypertension and chronic kidney disease: the ox or the plow?

Volume 80, Issue 10, Pages (November 2011)

Glomerular disease related to anti-VEGF therapy

John P. Middleton, Patrick H. Pun Kidney International

Volume 77, Issue 1, Pages 6-8 (January 2010)

An Uncommon Cause of Membranous Glomerulonephritis

Peritubular capillaritis in the renal allograft takes center stage

Donor kidney biopsies: pathology matters, and so does the pathologist

Volume 77, Issue 9, Pages (May 2010)

Medullary amyloidosis associated with apolipoprotein A-IV deposition

Volume 77, Issue 1, Pages 5-6 (January 2010)

Infection and chronic allograft dysfunction

Erik H. Strøm, Michael J. Mihatsch Kidney International

Comorbidity and confounding in end-stage renal disease

Volume 74, Issue 3, Pages (August 2008)

Lipid redistribution in renal dysfunction

Intra-glomerular metastases

Volume 70, Issue 11, Pages (December 2006)

Volume 54, Issue 2, Pages (August 1998)

The role of protein kinase C activation in diabetic nephropathy

Renal vascular sclerosis is associated with inherited thrombophilias

Harnessing regulatory T cells for therapeutic purposes

Infected glomerulus Kidney International

Volume 76, Issue 5, Pages (September 2009)

Response to ‘Hepcidin levels in patients with renal disease’

Macrophages and hypoxia in human chronic kidney disease

Banu Sis, Kim Solez, David Rayner Kidney International

Volume 85, Issue 3, Pages (March 2014)

C3 Glomerulonephritis: A Rare Etiology of the Pulmonary Renal Syndrome

Volume 69, Issue 12, Pages (June 2006)

Volume 69, Issue 3, Pages (February 2006)

Volume 85, Issue 5, Pages (May 2014)

Urinary biomarkers: the future looks promising

Volume 74, Issue 8, Pages (October 2008)

Alternate-day dialysis may be needed for hemodialysis patients

Nephrology Crossword: Peritoneal Dialysis

AIDS, nephrotic-range proteinuria, and renal failure

Immunofluorescence on pronase-digested paraffin sections: A valuable salvage technique for renal biopsies S.H. Nasr, S.J. Galgano, G.S. Markowitz, M.B.

Nephrology Crossword: Glomerulonephritis

Lipid-binding proteins in rat and human kidney

Volume 82, Issue 4, Pages (August 2012)

Volume 73, Issue 9, Pages (May 2008)

Volume 82, Issue 9, Pages (November 2012)

Volume 81, Issue 12, Pages (June 2012)

Volume 80, Issue 10, Pages (November 2011)

Volume 83, Issue 4, Pages (April 2013)

David A. Ferenbach, David C. Kluth, Jeremy Hughes Kidney International

Volume 70, Issue 3, Pages (August 2006)

The more or less ‘pristine’ renal allograft biopsy

The Case ∣ An Asian boy with proteinuria

Innovative teaching tools in nephrology

The international realities of live donor kidney transplantation

Tissue protection by erythropoietin: new findings in a moving field

Volume 80, Issue 9, Pages (November 2011)

Volume 74, Issue 10, Pages (November 2008)

Volume 75, Issue 8, Pages (April 2009)

Glomerular monocytes predict worse outcomes after acute renal allograft rejection independent of C4d status Kathryn J. Tinckam, Ognjenka Djurdjev, Alex.

Unmasking a unique glomerular lesion

Accepting prospective kidney donors with asymptomatic urinary abnormalities: Are we shooting in the dark? N. Vadivel, A. Stankovic, H.G. Rennke, A.K.

Morphology of the heart and arteries in renal failure

The Ebf1 knockout mouse and glomerular maturation

Ronen Levi, Justin Silver Kidney International

Epidemiologic data of renal diseases from a single unit in China: Analysis based on 13,519 renal biopsies Lei-Shi Li, Zhi-Hong Liu Kidney International

Alex B. Magil, Kathryn Tinckam Kidney International

Presentation transcript:

Tissue proteomics: a new investigative tool for renal biopsy analysis John R. Sedor Kidney International Volume 75, Issue 9, Pages 876-879 (May 2009) DOI: 10.1038/ki.2009.54 Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 1 Electron micrograph showing intramembranous electron-dense deposits with ribbon-like thickening of glomerular capillary walls (arrow). The mesangium is expanded by increased matrix and cells. Kidney International 2009 75, 876-879DOI: (10.1038/ki.2009.54) Copyright © 2009 International Society of Nephrology Terms and Conditions

Figure 2 Work flow for tissue proteomics. A kidney biopsy is formalin-fixed and paraffin-embedded (FFPE) by appropriate protocols. Kidney tissue sections are then processed for either histopathological evaluation or tissue proteomics. The kidney structures of interest are laser-microdissected; proteins are extracted and then subjected to mass spectrometry methodologies for identification. Kidney International 2009 75, 876-879DOI: (10.1038/ki.2009.54) Copyright © 2009 International Society of Nephrology Terms and Conditions