Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human preimplantation embryos Hongbo Wang, M.D., Yan Wen, M.D.,

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Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human preimplantation embryos Hongbo Wang, M.D., Yan Wen, M.D., Stephen Mooney, M.D., Hao Li, M.D., Barry Behr, Ph.D., Mary Lake Polan, M.D., Ph.D., M.P.H. Fertility and Sterility Volume 80, Pages 736-742 (September 2003) DOI: 10.1016/S0015-0282(03)00782-9

FIGURE 1 ET-PCR analysis of MMP and TIMP genes expressed in single human embryos. Agarose gels were stained with ethidium bromide showing electrophoretic bands corresponding to the cDNA amplification products derived from the second PCR amplification using MMP-1, MMP-2, MMP-9, TIMP-1, TIMP-2, and TIMP-3 inner primers. The β-actin transcript also was amplified to ensure the presence of intact mRNA. Wang. Human embryo MMP and TIMP expression. Fertil Steril 2003. Fertility and Sterility 2003 80, 736-742DOI: (10.1016/S0015-0282(03)00782-9)

FIGURE 2 Immunohistochemical localization of MMP-1 and TIMP-1 protein in a human blastocyst. Expanded blastocyst with positive staining for MMP-1 (A) and TIMP-1 (B) in inner cell mass (ICM) and trophectoderm cells (TE). (C) Negative control. Wang. Human embryo MMP and TIMP expression. Fertil Steril 2003. Fertility and Sterility 2003 80, 736-742DOI: (10.1016/S0015-0282(03)00782-9)