Fig. 1 ee cells are specified by ISCs asymmetric divisions.

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Fig. 1 ee cells are specified by ISCs asymmetric divisions. ee cells are specified by ISCs asymmetric divisions. (A) Numbers of ISCs (Prospero- esg>GFP+) and ee cells (Prospero+) during pupal development. Each data point represents one pupal midgut. Curves were generated by connecting adjacent average value positions. (B) Pros staining at 44 hours APF. Here and in all images, blue indicates 4′,6-diamidino-2-phenylindole (DAPI) and red indicates Pros; green indicates esg>GFP except where otherwise specified. (C and E) Schematic representation of wild-type MARCM clones and their outcomes induced at 36 hours APF (C) or 48 hours APF (E) and examined at 96 hours APF. (D) A four-cell clone (green) induced at 36 hours APF contains two Pros+ cells and two ISCs [arrowhead in (D′)]. (F and G) Two-cell clones induced at 48 hours APF contain either two ISCs (Pros–) (F) or two ee cells (Pros+) (G). (H to N) Pros asymmetrically localizes to the basal side during ISC mitosis. The apical daughter gradually moves toward the basal side during anaphase [(J) and (K)] and telophase [(L) to (N)]. The apical daughter extends a projection toward the basal side during telophase [arrowhead in (L) and (M)]. Insets in (H) to (N), DAPI; insets in (I′), (M′), and (N′), α-tubulin. Scale bars, 10 μm. Zheng Guo, and Benjamin Ohlstein Science 2015;350:aab0988 Copyright © 2015, American Association for the Advancement of Science