Centrosome anomalies characterize colorectal cancer with rhabdoid phenotype. Centrosome anomalies characterize colorectal cancer with rhabdoid phenotype.

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Centrosome anomalies characterize colorectal cancer with rhabdoid phenotype. Centrosome anomalies characterize colorectal cancer with rhabdoid phenotype. A, Histopathologic images from a subset of five additional prototypical rhabdoid colorectal cancers (RC), in which are evident rounded eosinophilic cytoplasmic inclusions, eccentric nuclei, and prominent nucleoli. Scale bar, 20 μm H&E images. B, Mutations for selected driver genes and CpG island methylation (CIMP) profile accompanied by loss of heterozygosity analysis at 1p36.13 locus. CROCC mRNA (qPCR) expression levels in tumors and adjacent normal mucosa. Data are mean ± SEM; (n = 5 biological replicates; *, P < 0.05; **, P < 0.01; two-tailed Student t test). C, Representative IHC analysis from case RC5: Cytokeratin-18 (CK18) marks intermediate filaments in an anucleated cell (arrow); Ki67 reveals abnormal chromosome structures (arrow); CROCC marks a multinucleated cell (arrow), anucleated cell (arrow) or it appears fragmented in a mitotic cell (monopolar spindle, arrow). Scale bar, 10 μm. Right, quantification of the centrosome phenotypes against CROCC observed in all RCs (n = 2 experiments, >500 cells/sample). D, Cytogenetic abnormalities (tetraploid signals, arrows) observed by FISH using the centromeric chromosome probes illustrated. Scale bars, 20 and 40 μm. Left, ploidy pattern in all RCs (for chromosomes 1, 12, and 17, n = 2 experiments, >500 cells per sample). Right, quantification of cells with polyploidy measured as ratio of triploid and tetraploid on diploid cells for each tumor. Andrea Remo et al. Mol Cancer Res 2018;16:1385-1395 ©2018 by American Association for Cancer Research