Single-Molecule Approaches Embrace Molecular Cohorts

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Presentation transcript:

Single-Molecule Approaches Embrace Molecular Cohorts Taekjip Ha Cell Volume 154, Issue 4, Pages 723-726 (August 2013) DOI: 10.1016/j.cell.2013.07.012 Copyright © 2013 Elsevier Inc. Terms and Conditions

Figure 1 Single-Molecule Approaches Dissect Multimolecule Behaviors (A) Protein 1 (P1) bound to DNA dissociates slowly unless nearby Protein 2 (P2) facilitates P1 dissociation or exchange. Facilitated dissociation may occur via microdissociation or multivalent interactions. (B) A tandem repeat protein made of two domains, one with A and B motifs and the other with A′ and B′ motifs, may fold properly into AB-A′B′ or misfold into AB′-A′B. Interdomain misfolding can be significant if the sequence identity between the two domains is high. (C) Six different conformations of two tandem domains of calmodulin are illustrated, along with their connectivity. Folded motifs are denoted as semicircles, and unfolded motifs are denoted as curves. (D) A single UvrD protein translocates on ssDNA and stops when it encounters a junction with dsDNA. When a second UvrD joins, the two proteins can unwind the DNA processively. Kymogram shows UvrD position as a function of time, and the brightness is a measure of UvrD stoichiometry. The force versus time curve shows that DNA shortening due to conversion of dsDNA into ssDNA initiates upon the arrival of a second UvrD. Reproduced from Lee et al. (2013) with permission from Nature Publishing Group. Cell 2013 154, 723-726DOI: (10.1016/j.cell.2013.07.012) Copyright © 2013 Elsevier Inc. Terms and Conditions