Protothecosis and Prototheca spp.

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Protothecosis and Prototheca spp. Protothecosis and Prototheca spp. (A) Histopathology by hematoxylin and eosin staining of cutaneous protothecosis shows morula-like structures (large arrow) and endospores (small arrow). Magnification, ×1,000. (B) Typical morphology is best observed with PAS. (C and D) Wet mount preparation with lactophenol cotton blue shows P. zopfii, with asymmetrical morula-like structures (C), and P. wickerhamii, with symmetrical morula-like structures (D). (E) Smooth, creamy, white, yeastlike colonies of P. zopfii on Columbia blood agar after incubation at 37°C for 24 h. (F) Staining of bronchoalveolar lavage fluid with calcofluor white also yields typical morula-like structures of P. zopfii. Cornelia Lass-Flörl, and Astrid Mayr Clin. Microbiol. Rev. 2007; doi:10.1128/CMR.00032-06