Mechanism of action studies using paenimucillin C (4F).

Slides:

Advertisements

Similar presentations

Triacylglycerol Synthesis

Advertisements

If fatty acid synthesis occurs in the cytosol, where does the acetyl-CoA come from? Acetyl-CoA (not from fatty acid oxidation) is generated in the mitochondria.

Glycerolipid and Glycerophospholipid biosynthesis Vasiliy Portnoy, UCSD Graduate program in bioengineering Introduction Major constituents of the bacterial.

Phospholipid A phospholipid is a type of lipid used in the cells of living things.

Phospholipid Bilayer. The Cell Membrane a phospholipid bilayer.

COMPLEX LIPIDS DENTAL BIOCHEMISTRY 2015 Lecture 18 Michael Lea.

Lipid Synthesis Prof S. Kajuna

BRC Science Highlight Mechanism of Ionic Liquid Toxicity and Rational Engineering of a Tolerant Yeast Strain Objective To identify genetic determinants.

Reactive oxygen species and membrane permeability assay.

Volume 9, Issue 2, Pages (February 2009)

September 2014.

FAT SIGNALS - Lipases and Lipolysis in Lipid Metabolism and Signaling

Cell Membrane Notes.

Summary of genetic alterations in resistant biopsies among patients progressing on ceritinib or alectinib. Summary of genetic alterations in resistant.

The Plasma Membrane.

Schematic view of SGI1-L and its specific features encountered in Morganella morganii strain LIM90. Schematic view of SGI1-L and its specific features.

Volume 17, Issue 5, Pages (May 2013)

Mutations in LPIN1 Cause Recurrent Acute Myoglobinuria in Childhood

Lipid biosynthesis. Lipid biosynthesis. Schematic overview of the pathways involved in the synthesis of fatty acids (FAs), cholesterol, phosphoglycerides,

Disruption of the BGLF2 gene decreased infectious progeny production.

Genomic rearrangements of E

Putative ammonia transporters are not required for ammonia release during N-acetylglucosamine pH neutralization. Putative ammonia transporters are not.

Phospholipids and Membrane Structure

TEM on spore section of strain ATCC 9714.

Personalized Medicine: Patient-Predictive Panel Power

Ions representative of bronchial tissue (m/z 88

Figure 5 Ether-containing lipid biosynthesis pathways

Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. Phylogeny of Shiga toxin-encoding phage from SDi/SJo S. sonnei isolates. (A) progressiveMauve.

SEM images of S. aureus ATCC cells left untreated (A) or treated with polymyxin B (PMB; 99.5 mg/liter) (B), FADDI-019 (1× MIC) (C), FADDI-019 (4×

Johannes A. Mayr, Tobias B. Haack, Elisabeth Graf, Franz A

Spores of strain ATCC 9714 imaged by SEM (A), cryo-EM (B), and 3D-SIM (C and D) with spores labeled with lipid dyes DiO and FM 4-64FX (C) or DiO and spore-specific.

Polymorphism discovery in 09-CB1 × IPO323 versus 09-ASA-3apz × IPO94269 bulks. Polymorphism discovery in 09-CB1 × IPO323 versus 09-ASA-3apz × IPO94269.

Membrane cytoskeleton: PIP2 pulls the strings

RAD51 is essential for L. donovani.

Adapted viruses replicate in mismatched miR-122-expressing cells.

Phylogenetic tree of 170 Lactobacillus species.

Cell wall hydrolytic activities of Andhra lysins.

Time-lapse microscopy of a growing single cell with fluorescently labeled cell wall. Time-lapse microscopy of a growing single cell with fluorescently.

FLC treatment results in an increase in ploidy in a significant fraction of cells. FLC treatment results in an increase in ploidy in a significant fraction.

A Dynamic Interface between Vacuoles and Mitochondria in Yeast

Capture and ejection of inclusion bodies by minicells.

Side-view and apical-view projections of mutant and validation strain biofilms. Side-view and apical-view projections of mutant and validation strain biofilms.

Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant with Triton X-100. Relative growth of WT R2866 and the R2866ΔampG gene deletion mutant.

Stress-protective roles of C. auris Hog1.

RNA-Seq analysis of CYR1 cells in the adaptation to acid pH.

FAT SIGNALS - Lipases and Lipolysis in Lipid Metabolism and Signaling

General structure of RIFINs and STEVORs

Lipid mass spectrometry analysis of WT, tgl3Δ and vps4Δ mutant cells.

E. coli CFT073 and E. coli MG1655 persistence.

The original syn-BNPs (paenimucillin A [1F] and B [1W]) were synthesized according to Minowa and NRPSPredictor2, two algorithms commonly used for prediction.

SAM transport by Gap4 in S. cerevisiae cells.

Uptake of amino acids by C. albicans.

Distribution of signaling molecules in a section of P

Bacterial composition of olive fermentations is affected by microbial inoculation. Bacterial composition of olive fermentations is affected by microbial.

ITS rRNA gene locus. ITS rRNA gene locus. Schematic of the eukaryotic ribosomal gene cluster. The SILVA database contains sequences of the 18S gene, while.

Neonatal HSV-2 genomes are genetically distinct from one another and encompass a broad range of known HSV-2 genetic diversity. Neonatal HSV-2 genomes are.

PLase cleavage sites in phosphatidylcholine and cellular activities of the reaction products. PLase cleavage sites in phosphatidylcholine and cellular.

Stress resistance comparisons.

Septin ring formation and chitin-containing septum formation are aberrant in filaments formed in response to staurosporine. Septin ring formation and chitin-containing.

Distribution of the diversity numbers of antibiotic resistance and virulence factor protein families by environmental metagenomes’ protein family richness.

Mammalian cell cytotoxicity shown by macrophage response to DM262.

Normalized abundance of tetracycline resistance genes in Swedish infants by AMR++. Normalized abundance of tetracycline resistance genes in Swedish infants.

Mig1 influences the TOR and cell wall remodeling pathways.

Volume 63, Issue 6, Pages (September 2016)

Distribution of selected traits across the >4,000 strains in the most recent version of the database, including cell shape (A), spore formation (B), motility.

Growth with N-acetylglucosamine rapidly raises the environmental pH and releases detectable ammonia. Growth with N-acetylglucosamine rapidly raises the.

Phylogenetic analysis of complete Fusobacterium genomes.

GBM 112 and GBM 144 cells infected in vitro with HCMV resist growth inhibition by temozolomide. GBM 112 and GBM 144 cells infected in vitro with HCMV resist.

Batch variation of formulations from two products by two different genomic-scale techniques. Batch variation of formulations from two products by two different.

Prediction of cross-resistance among rifampin-resistant isolates.

Presentation transcript:

Mechanism of action studies using paenimucillin C (4F). Mechanism of action studies using paenimucillin C (4F). (a) Among strains resistant to 4F, genome sequencing identified common mutations in three genes (pla1, pld, and pgsA) within the cell membrane phospholipid metabolism network. Abbreviations: CDP-DAG, CDP diacylglycerol, CL, cardiolipin; G3P, glycerol-3-phosphate; LPA, lysophosphatidic acid; PA, phosphatidic acid; PS, phosphatidylserine. Panels b and c show results from permeability assays using DIBAC4 (b) and SYTOX (c), fluorescent dyes for probing membrane depolarization and pore formation, respectively. Polymyxin and Triton X-100 serve as positive controls for membrane depolarization and pore formation, respectively. Xavier Vila-Farres et al. mSphere 2018; doi:10.1128/mSphere.00528-17