Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a.

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Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. Confirmation of spliced RNA in cells transfected with a wild-type or Pol(−) MR766 ZIKV plasmid. (A) RT-PCR products from RNA from cells transfected with the wild-type or Pol(−) MR766 ZIKV plasmid or infected with the parental MR766 virus. Band sizes (kilobases) are indicated to the left of the gel. Two product sizes were observed, representing the unspliced (1,083-bp) and spliced (1,216-bp) template. (B) Sequence results of RT-PCR products from transfected or infected cells aligned to three reference sequences: parental MR766 virus, unspliced plasmid, and spliced plasmid. Megan C. Schwarz et al. mSphere 2016; doi:10.1128/mSphere.00246-16