Fig. 5. Can f 4-specific mAbs and the sandwich ELISA for the quantification of Can f 4. (A) The epitope specificity of the three Can f 4-specific mAbs.

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Fig. 5. Can f 4-specific mAbs and the sandwich ELISA for the quantification of Can f 4. (A) The epitope specificity of the three Can f 4-specific mAbs analyzed by competitive ELISA. Binding of the biotinylated mAbs to nCan f 4 was inhibited with the same mAbs which were not biotinylated. Percentages of inhibition are indicated. (B) The linear part of the recombinant Can f 4 standard curve is between 10 and 1,000 ng/mL in the sandwich ELISA. (C) Can f 4 concentrations of experimental home dust samples measured by the mAb-based sandwich ELISA. Horizontal lines represent the mean values. Fig. 5. Can f 4-specific mAbs and the sandwich ELISA for the quantification of Can f 4. (A) The epitope specificity of the three Can f 4-specific mAbs analyzed by competitive ELISA. Binding of the biotinylated mAbs to nCan f 4 was inhibited with the same mAbs which were not biotinylated. Percentages of inhibition are indicated. (B) The linear part of the recombinant Can . . . Allergy Asthma Immunol Res. 2015 Jul;7(4):384-392. http://dx.doi.org/10.4168/aair.2015.7.4.384