Pulmonary Artery Leiomyosarcoma Expressing Epstein-Barr Virus in an Immunocompetent Individual Sana Yokoi, MD, PhD, Toshihiko Iizasa, MD, PhD, Kenzo.

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Pulmonary Artery Leiomyosarcoma Expressing Epstein-Barr Virus in an Immunocompetent Individual Sana Yokoi, MD, PhD, Toshihiko Iizasa, MD, PhD, Kenzo Hiroshima, MD, PhD, Yukio Saitoh, MD, PhD, Takehiko Fujisawa, MD, PhD The Annals of Thoracic Surgery Volume 81, Issue 5, Pages 1897-1899 (May 2006) DOI: 10.1016/j.athoracsur.2005.05.083 Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 (A) Computed tomographic scan showed a right central pulmonary artery obstruction with extension to the pulmonary trunk by an inhomogeneous tumor, and (B) multiple sphenoid nodules in subpleural regions of the middle and lower lobe of the right lung. The Annals of Thoracic Surgery 2006 81, 1897-1899DOI: (10.1016/j.athoracsur.2005.05.083) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Results of polymerase chain reaction amplification of a 140 base pair product derived from the BamH I W fragment of Epstein-Barr virus performed on 5 DNA samples by agarose gel electrophoresis. Samples were derived from resected tumor (lane 1) and normal lung tissue (lane 2) from the patient presented in this report, pulmonary artery leiomyosarcoma (lane 3) and normal lung tissue (lane 4) from an autopsy sample from a different patient, another pulmonary artery leiomyosarcoma autopsy from a different patient (lane 5), and the Raji cell line as a positive control (lane 6). The primers W-1 (5’-GTTCGCGTTGCTAGGCCACC-3’) and W-2b (5’-TGGCGCTCTGATGCGACCAG-3’) were used from previously reported conditions (40 cycles, annealing at 57°C for 2 minutes) [2]. The Annals of Thoracic Surgery 2006 81, 1897-1899DOI: (10.1016/j.athoracsur.2005.05.083) Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions