Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs

Slides:

Advertisements

Similar presentations

A. a). d). c). f). b).e). g). Figure S1. NAT2 expression in lymphocytes and monocytes from a healthy volunteer. PBMC from a healthy volunteer were simple.

Advertisements

Retrovirally Transduced CD34++ Human Cord Blood Cells Generate T Cells Expressing High Levels of the Retroviral Encoded Green Fluorescent Protein Marker.

Volume 125, Issue 2, Pages (August 2003)

Representative flow cytometry dot plots from a healthy individual

Melanoma donor (MD) NK cells are functionally impaired/exhausted.

by Adam M. Zawada, Kyrill S

Cell-to-cell contact between activated CD4+ T lymphocytes and unprimed monocytes interferes with a TH1 response Miriam Wittmann, MD, Mareike Alter, Tanja.

Further Analysis of Interleukin-2 Receptor Subunit Expression on the Different Human Peripheral Blood Mononuclear Cell Subsets by Denis David, Lynda Bani,

FOXP3+ CD4 T-cell maturity and responses to microbial stimulation alter with age and associate with early-life gut colonization Sophia Björkander, Maria.

Preformed CD40 ligand exists in secretory lysosomes in effector and memory CD4+ T cells and is quickly expressed on the cell surface in an antigen-specific.

Hydroxychloroquine inhibits calcium signals in T cells: a new mechanism to explain its immunomodulatory properties by Frederick D. Goldman, Andrew L. Gilman,

by Ulrike Schleicher, Andrea Hesse, and Christian Bogdan

TIGIT expression in naive T cells is accelerated by coculture with si-tolerant T cells at an early-stimulation stage. TIGIT expression in naive T cells.

Exposure to rSema4A increases Treg cell numbers in human PBMC in vitro cultures. Exposure to rSema4A increases Treg cell numbers in human PBMC in vitro.

Human Treg cells express PlexinB1.

Effect of quercetin on colony-forming abilities of prostate cancer cell lines with different aggressive potentials. Effect of quercetin on colony-forming.

SpA-SEA-D227A-induced CTL lyse MAb-coated EC

Exogenous IL-4 cannot rescue Th2 cytokine expression in HuR-deficient cells. Exogenous IL-4 cannot rescue Th2 cytokine expression in HuR-deficient cells.

Loss of pathogen-specific T cell memory is due to the absence of Runx2 in CD8+ T cells. Loss of pathogen-specific T cell memory is due to the absence of.

The K594ScFv-SEA-D227A fusion protein targets CTL to lyse EA

Effect of OPN on apoptosis of DCs

Ex vivo IL-6 production. Ex vivo IL-6 production. Human whole blood was stimulated with the indicated doses of the E. coli O113 endotoxin that was used.

DCs induce the proliferation of autologous T cells.

Figure S1. Representative gating of 7-hr suppression assay from one subject Teff with media alone (top row), Teff with activation beads but not Tregs (middle),

Proinflammatory activities of Lactobacillus subcellular fractions.

Flow cytometry analysis of TNF-β- and IL-10-producing CD33+ cells.

Volume 125, Issue 2, Pages (August 2003)

Hematological alterations after endotoxin injection.

Volume 11, Issue 11, Pages (June 2015)

Profiles of anti-inflammatory cytokines after endotoxin injection.

IRF4 is required for efficient Th1 CD4+ effector cell differentiation.

Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β in milk. Effects of E. coli and S. aureus infection on levels of TNF-α and IL-1β.

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

(A) CDK2 and p53 gene expression levels, as quantitated by real-time quantitative PCR with SYBR Green master mix (Stratagene, La Jolla, Calif.). (A) CDK2.

IRF4 is required for efficient Th1 CD4+ effector cell differentiation.

CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to mitogen. CD27 expression on bovine γδ and CD4 T cells ex vivo and in response to.

The risk allele of rs affects TCR-mediated intracellular calcium flux in CD4+ T cells from subjects with T1D or RA. Previously frozen PBMCs from.

Comparison of conventional NSG and hIL-7 expressing NSG humanized mice

CD151-knockdown cells show an aberrant spreading response to PMA

Loss of BAP1 blocks T cell differentiation at the DN3 stage in vitro.

IL-6 production with increasing doses of endotoxin.

Intracellular TNF-α and IL-4 expression in gated CD33+ monocytes by three-color flow cytometry. Intracellular TNF-α and IL-4 expression in gated CD33+

Flow cytometric analysis of T cell activation.

pDCs from the melanoma environment responded to TLR-L stimulation.

The NTP increases tyrosine phosphorylation of PLC-γ1.

Binding and internalization of bispecific immunotoxins.

Comparison of OPN production by mature DCs and activated macrophages.

OPN mediates maturation from immature DCs to mature DCs

Reactivity of the anti-p36 MAbs against whole M

Flow cytometry analysis of intracellular cytokine expression in control PBMC (left set of panels) and following either PMA-ionomycin activation (middle.

Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children (n = 50; B). Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children.

ALT-803 stimulates proliferation and activation of human NK cells and T cells in vitro. ALT-803 stimulates proliferation and activation of human NK cells.

Representative cytokine and cytotoxicity responses to CMV peptides on day 0 and day 7 in one HLA A2+ donor. Representative cytokine and cytotoxicity responses.

Effect of HL60 cell age (expressed in cell passage) on opsonophagocytic activity. Effect of HL60 cell age (expressed in cell passage) on opsonophagocytic.

IgM-specific immunoblot analysis of different HCMV recombinant antigens with a selected serum sample from an HCMV-seronegative patient with acute EBV-induced.

PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate distinct mechanisms of regulation. PD-1 and CD103 are coexpressed on CD8+ T cells but demonstrate.

Dot plot comparison of EBNA-1 with recombinant antigens 52/3 (aa 297 to 433 of pUL44) and UL57/3 (aa 545 to 601 of pUL57) of HCMV, containing glycine-rich.

Effects of intramammary infection with E. coli or S

Flow cytometric gating strategy used to identify CD69 surface expression on CD4 (left paired columns) and CD8 (right paired columns) T lymphocytes. Flow.

LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. LDL cholesterol inhibits Vγ9Vδ2 T-cell activation and cytokine production. Preexpanded.

Typical pattern of the degranulation of NK cells from a healthy donor (upper row) and a patient with FRH in recurrence (middle row) and in remission (lower.

Fig. 5 IL-5–mediated signaling is critical for the development of CD1dintCD5+ Breg precursor cells and IL-10+ Breg cells. IL-5–mediated signaling is critical.

Memory phenotypes PBMC-derived MERS-CoV-specific T cells from CWs

Quantitation of apoptosis in M. aviumsubsp

Immunoblot of scFv antibody detected with an anti-FLAG antibody.

OPN production by immature DCs and mature DCs treated with PAMPs

Mean reciprocal IFA titer results for serum samples from experimentally vaccinated animals. Mean reciprocal IFA titer results for serum samples from experimentally.

The NTP inhibits the AFC response to SRBC

Indirect immunofluorescence reactions on primate smooth muscle tissue for EMA detection depicting the following: (A) prozone reactions at low dilution.

Presentation transcript:

Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs. PBMCs were cultured for 6 h in medium (left panels) or were stimulated with PMA-ionomycin (middle panels) or PHA (right panels) in the presence of monensin. Surface CD4 and CD14 and intracellular TNF-α expression in PBMCs. PBMCs were cultured for 6 h in medium (left panels) or were stimulated with PMA-ionomycin (middle panels) or PHA (right panels) in the presence of monensin. Dot plots of CD4-FITC (FL1 [green fluorescence]) (A) or CD14-FITC (B) versus TNF-α–PE (FL2 [orange fluorescence]) expression in the whole PBMC population are shown. Numbers show percentages of positive cells. Data from one representative experiment of three experiments performed are shown. Jarołsaw Baran et al. Clin. Diagn. Lab. Immunol. 2001; doi:10.1128/CDLI.8.2.303-313.2001