Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor.

Slides:

Advertisements

Similar presentations

Identification of combination treatment–responsive effector/memory tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Advertisements

Identification of combination treatment–responsive dysfunctional tumor-infiltrating CD8+ T cell population. Identification of combination treatment–responsive.

Three different types of transfer functions with a codomain of [0,1].

Resistance of CD141+ DCs to influenza virus in vivo in humanized mice.

Fig. 3 CSF1 is expressed in human melanoma.

Protection from autoimmunity is not due to the expansion of Treg subsets. Protection from autoimmunity is not due to the expansion of Treg subsets. (A.

Platelets are required for hFcγRIIA-induced anaphylaxis.

Human cells produce type I and III IFNs upon Af stimulation.

β-Glucans do not modulate epithelial IL-33 or AHR.

Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation.

Fig. 7 Correlation of NHP and human ISGs.

Fig. 5 Putative latency-reversing agents exert differential effects on the different blocks to HIV transcription. Putative latency-reversing agents exert.

Differentiation of AZD4785 from MAPK pathway inhibitors in vitro

Fig. 6 In utero injection of inflammatory cytokines or adoptive transfer of activated T cells leads to pregnancy loss. In utero injection of inflammatory.

IL-10R-deficient macrophages secrete IL-23, inducing IL-22 secretion by ILC3 and TH17 cells. IL-10R-deficient macrophages secrete IL-23, inducing IL-22.

Mammary cell proliferation is inhibited when LAD1 is depleted.

Fig. 4 Labeling of Msmeg with DMN-Tre is fast and specific and depends on Ag85A function. Labeling of Msmeg with DMN-Tre is fast and specific and depends.

Fig. 2 AcPGP induces IL-8 and G-CSF release from human bronchial epithelial cells. AcPGP induces IL-8 and G-CSF release from human bronchial epithelial.

Cell viability tests. Cell viability tests. SEM images of (A) MC3T3-E1 cells and (B) MSCs on days 1, 3, and 5 of culture. (C) Survival rates of MC3T3-E1.

Fig. 4. PVSRIPO infection of DCs is sublethal, is marginally productive, and induces sustained proinflammatory cytokine production. PVSRIPO infection of.

Fig. 3 M7824 inhibits tumor growth and metastasis and provides long-term antitumor immunity. M7824 inhibits tumor growth and metastasis and provides long-term.

Fig. 8 Combining M7824 with radiation or chemotherapy enhances antitumor efficacy. Combining M7824 with radiation or chemotherapy enhances antitumor efficacy.

Fig. 5 Cell-free membrane cyclase assay confirms that candidate compounds are specific antagonists of hNPR1. Cell-free membrane cyclase assay confirms.

Blood Tfr cells do not show specialized humoral regulatory capacity.

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

Fig. 3. Increased expression of exhaustion markers and apoptosis markers on CAR8 cells in the presence of TCR antigen. Increased expression of exhaustion.

Fig. 1. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy. CAR4 and CAR8 cells demonstrate in vitro and in vivo antileukemic efficacy.

Fig. 1 Inbred mouse strains carrying monoclonal tumors display a symmetrical yet disparate response to ICB, associated with a distinctive gene signature.

Fig. 4 Administration of BMS to mice inhibits autoimmune-relevant pharmacodynamic endpoints driven by IL-12 and IFNα. Administration of BMS

Fig. 4 Rational therapeutic modulation of the tumor microenvironment sensitizes tumors to ICB. Rational therapeutic modulation of the tumor microenvironment.

Fig. 3 BMS blocks functional responses in primary immune cells driven by IL-23 and IL-12. BMS blocks functional responses in primary immune.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fig. 7 BMS reduces the elevated expression of type I IFN–regulated genes both ex vivo in blood from patients with lupus and in a phase 1 study of.

Fig. 2 In situ vaccination of CpG in combination with anti-OX40 antibody cures established local and distant tumors. In situ vaccination of CpG in combination.

BMS blocks functional responses in primary immune cells driven by IFNα

Fig. 5 Treatment with BMS (PO BID) protects from wasting and colitis in two SCID mouse models. Treatment with BMS (PO BID) protects from.

Fig. 3 Identification of KEECs that increase KCC2 expression in human RTT neurons. Identification of KEECs that increase KCC2 expression in human RTT neurons.

Fig. 1 Genes up-regulated in Zic5 KO cells correlate with the increased glycolytic state. Genes up-regulated in Zic5 KO cells correlate with the increased.

In vitro cell-release experiments on a glass substrate.

Fig. 2 ODN enhances EV transfer between cells expressing TLR9.

S-Affs colocalize with SUMO in mammalian cells.

Gab3 is required for IL-2– and IL-15–induced priming and expansion of NK cells. Gab3 is required for IL-2– and IL-15–induced priming and expansion of NK.

Fig. 5 n-HA regulates gene expressions related to tumor suppression, calcium homeostasis, and immune response. n-HA regulates gene expressions related.

Fig. 2 Extended local release of agonists of innate immunity prevents tumor recurrence and eliminates distal metastases. Extended local release of agonists.

Fig. 1. Matrix stiffness sensitizes myofibroblasts to apoptosis induced by inhibition of their mechanotransduction pathways. Matrix stiffness sensitizes.

Fig. 3 Local Maraba treatment of TNBC tumors provides long-term systemic protection. Local Maraba treatment of TNBC tumors provides long-term systemic.

Fig. 2 ObR is cleaved and has reduced signaling capabilities after incubation with Mmp-2. ObR is cleaved and has reduced signaling capabilities after incubation.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

Immune evasion occurs independently of perforin-mediated killing.

Fig. 5. Vascularization of human liver seed grafts.

Fig. 3. Human liver tissue seed graft function.

Fig. 5 In vivo gene delivery by the T4-AAV nanoparticles.

Chronic Treg reduction results in dermal fibrosis.

Fig. 4 IP6K1-mediated polyP production by platelets plays a critical role in LPS-induced NPA formation. IP6K1-mediated polyP production by platelets plays.

Fig. 5 Increased myometrial cell contractility in response to fetal T cells from preterm infants. Increased myometrial cell contractility in response to.

Fig. 1 Study design and antibody pharmacokinetics.

Fig. 1 Undetectable ALK expression in monocytes and macrophages.

Fig. 7 BEL immune response.

Fig. 2 ITGA5 knockdown attenuates hPSC activation, differentiation, and functions. ITGA5 knockdown attenuates hPSC activation, differentiation, and functions.

Fig. 2. Mechanism of PD-L1 down-regulation in NOD HSPCs.

Fig. 3 Superiority of BAFF-R versus CD19-CAR T cells in a Burkitt lymphoma model is not due to greater tumor antigen density. Superiority of BAFF-R versus.

Fig. 5 The complex of antibody and dye can afford to target imaging in the NIR-II window. The complex of antibody and dye can afford to target imaging.

Fig. 1 Differentiation of human peripheral blood monocytes into MDMi cells induces a microglial gene expression and functional phenotype. Differentiation.

Fig. 3 MCA-mediated neutrophil recruitment accelerates bacterial clearance in the skin. MCA-mediated neutrophil recruitment accelerates bacterial clearance.

Fig. 1 Generation and characterization of MeV-based vaccine candidates for Lassa virus. Generation and characterization of MeV-based vaccine candidates.

Fig. 4 Acoustophoretic printing of food, optical, biological, and electrically conductive materials. Acoustophoretic printing of food, optical, biological,

Fig. 2 hTERT induces expression of heat shock protein genes through HSF1 and interacts with Hsp70-1. hTERT induces expression of heat shock protein genes.

Fig. 3 Correction of Dmd exon 44 deletion in mice by intramuscular AAV9 delivery of gene editing components. Correction of Dmd exon 44 deletion in mice.

Fig. 2. Cellular response to FolamiRs in vitro.

Cxxc1-deficient TH17 cells exhibit a Treg cell–like expression profile

Presentation transcript:

Fig. 4 Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. Rotavirus induces the type I IFN pathway and release of ATP by tumor cells. (A) List of the 23 shared genes commonly up-regulated by A20 and EMT6 cancer cell lines upon in vitro rotavirus exposure with at least 1.5 log2 fold change in expression and a P < 0.005 (mean values of triplicates). For the two log2 fold change columns, a gradient from white to red allows identification of the genes with the weakest (white) to the strongest (red) up-regulations. Genes highlighted in gray are in common with the list of genes up-regulated in A20 upon exposure with inactivated rotavirus (see Fig. 7D). (B) Pictures of immunofluorescence staining (magnification, ×20) with anti-MxA–specific monoclonal antibodies and secondary detection on A20 tumors harvested from Balb/c mice after either IT rotavirus or IT PBS treatment. Staining was performed on frozen tumor sections. An isotype control was used as a negative control for MxA detection (in green) after DAPI (4′,6-diamidino-2-phenylindole) counterstaining (in blue). The means (±SEM) of immunofluorescence intensity as measured by the ImageJ software are presented in the right panel. Data from n = 2 mice in each group with three tumor sections. Unpaired one-tailed t test, **P = 0.005. (C) ATP release in the cell culture supernatant upon several dilutions of rotavirus exposure on human (SH-SY5Y neuroblastoma) or murine (Neuro2a neuroblastoma and A20 B-cell lymphoma) tumor cell lines. Measurement performed by luminescence assay using CellTiter-Glo Luminescent Cell Viability Assay kit 48 hours after incubation with different dilutions of rotavirus. Results are reported as RLU intensity corresponding to relative ATP release intensity. Data as means ± SEM, n = 3 wells per dilution; one-way ANOVA test, ****P < 0.0001, ***P < 0.0005, and **P < 0.005. Tala Shekarian et al., Sci Transl Med 2019;11:eaat5025 Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works