Altered expression of a cell-cycle suppressor gene, Tob-1, in endometriotic cells by cDNA array analyses  Dan I Lebovic, M.D., Russell A Baldocchi, Ph.D., Michael D Mueller, M.D., Robert N Taylor, M.D., Ph.D.  Fertility and Sterility  Volume 78, Issue 4, Pages 849-854 (October 2002) DOI: 10.1016/S0015-0282(02)03319-8

FIGURE 1 Clontech Atlas expression array analysis of endometrial stromal cells treated with interleukin-1β (IL-1β). Poly-adenylated RNA was isolated from variously treated cells (detailed in Methods), labeled by reverse transcribing with [32P]dATP, and hybridized to filters spotted with cloned cDNA PCR products. Differential amounts of specific transcripts were determined by measuring normalized spot intensity variations. Arrows indicate spots that represent the Tob-1 transcript, the abundance of which varied as a function of both treatment with IL-1β and stromal cell type. The difference in Tob-1 expression method by this assay is indicated on the figure. NSC = normal stromal cells; ESC = endometriotic stromal cells. Lebovic. Altered cell-cycle gene in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 849-854DOI: (10.1016/S0015-0282(02)03319-8)

FIGURE 2 Northern analyses demonstrating the induction of Tob-1 mRNA. Representative Northern blots demonstrating the induction of Tob-1 mRNA in interleukin-1β (IL-1β)-treated (10 ng/mL) human normal stromal cells (NSC) and endometriotic stromal cells (ESC). Tob-1 transcripts of 2.4-kb were detected. The positions of 28S and 18S ribosomal RNA on the original gels are marked. The integrity and amount of total RNA loaded were confirmed by subsequent hybridization of the blot with a glyceraldehyde-3-phosphate dehydrogenase (GAPDH) probe (1.2-kb). Lebovic. Altered cell-cycle gene in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 849-854DOI: (10.1016/S0015-0282(02)03319-8)

FIGURE 3 PhosphorImaging quantification of Northern analyses for Tob-1 mRNA. The Tob-1 mRNA levels in interleukin-1β (IL-1β)-treated normal stromal cells (NSC) and endometriotic stromal cells (ESC) are shown overlapping (■) the percentage in untreated controls (□). Interleukin-1β inhibited Tob-1 mRNA levels <3% and 25% ± 5% in NSC and ESC, respectively. Lebovic. Altered cell-cycle gene in endometriosis. Fertil Steril 2002. Fertility and Sterility 2002 78, 849-854DOI: (10.1016/S0015-0282(02)03319-8)