3D Printed Mechanical Bioreactor Group members: Sharon Adkins, Jaiden Oropallo, Kai Clarke, and Deniz Yenigun

Problem Statement: Goal: Mechanical testing for tissue engineered constructs is often done by an instron or large scale bioreactor. The instron is not capable of accurately taking measurements for constructs smaller than 10mm in regards to tensile testing. As a result, a solution is needed to combat this issue, hence the need for a small scale bioreactor. Goal: To design and produce a low cost 3D printed bioreactor that is capable of conducting mechanical tests on constructs <10mm and also able to conduct these tests under a cell culture hood for sterility.

What is a Bioreactor: What is the Instron: A bioreactor is defined as a vessel that carries out a biological reaction and is used to culture aerobic cells for conducting cellular or enzymatic immobilization. What is the Instron: A machine used to test the mechanical properties of materials and components.

Approach Perform literature review to find the most compatible materials from which we will produce our 3D printed bioreactor as well as appropriate dimensions needed for individual parts. With the assistance from a CSE student we will decide which method is best for allowing our device to communicate with the Instron the school currently has. Design the aforementioned parts in CAD and begin the 3D printing process of the parts as well as assembly. Design and produce the constructs we will be testing (Most likely will be electrospun rather than 3D printed). Begin conducting validation tests using or bioreactor and comparing it to the values found in literature. Compiling our findings and winning the design showcase.

How a CSE Student Can Help We need a program that will be able to communicate with the Instron Machine to perform the same task, but on a smaller scale. Do we need a seperate program to accomplish this? How will we attach our device to the instron?

Contact Info Primary Contact: Dr. Kunal Mitra kmitra@fit.edu Group Memebers Sharon Adkins: sadkins2017@my.fit.edu Kai Clarke: kclarke2016@my.fit.edu Jaiden Oropallo: joropallo2016@my.fit.edu Deniz Yenigun: dyenigun2014@my.fit.edu